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Human Myoglobin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips.
|Sample Type:||Serum and Plasma|
|Assay Range:||25-1000 ng/ml|
The Bosterbio OneStep ELISA kit is a solid phase sandwich ELISA kit. Instead of incubating with standards, samples and controls and with enzyme conjugated detection antibody separately, the OneStep ELISA kit allows the user to add standards, samples and controls to wells without incubation, and an enzyme conjugate reagent is added into each well immediately. Then incubate standards, samples, controls and enzyme conjugate reagent together. After the excess enzyme conjugate is washed out, the substrate is added into each well. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of color developed is directly proportional to the concentration of target protein in the samples. A standard curve is generated relating color intensity to the concentration of target protein.
|Product Name||Human Myoglobin OneStep ELISA Kit
See more Myoglobin products
|Storage & Handling||Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human Myoglobin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips.|
|Cite This Product||Human Myoglobin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7069)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||Serum and Plasma|
|Assay Range||25-1000 ng/ml|
|Cross Reactivity||There is no detectable cross-reactivity.|
|NCBI Gene Id||4151|
|Species Of This Entry||Human|
|Alternative Names||Myoglobin|MB; MGC13548; Myoglobin; PVALB|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for MB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|1. Microwell coated with murine monoclonal anti-myoglobin.||12x8x1|
|2. Reference Standard Set||0.5 ml|
|3. Sample Diluent||25 ml|
|4. Enzyme Conjugate Reagent||22 ml|
|5. TMB Reagent||11 ml|
|6. Stop Solution||11 ml|
|7. Wash Concentrate 20x: 1 Bottle||25 ml|
This data is generated from a recent batch of EK7069 Human Myoglobin OneStep ELISA Kit. It is not necessarily the same data set used to generate the standard curve shown in the image of this product.
We measured random samples of Human Myoglobin OneStep ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human Myoglobin OneStep ELISA Kit to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
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