Human Myoglobin OneStep ELISA Kit

Product Name

Human Myoglobin OneStep ELISA Kit

See all MB products

SKU/Catalog Number

EK7069

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Myoglobin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Myoglobin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7069)

Clonality of Antibodies

See Datasheet for details

Sensitivity

25 ng/ml

Assay Range

25-1000 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7069 is reactive to MB in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7069 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
1. Microwell coated with murine monoclonal anti-myoglobin.	12x8x1
2. Reference Standard Set	0.5 ml
3. Sample Diluent	25 ml
4. Enzyme Conjugate Reagent	22 ml
5. TMB Reagent	11 ml
6. Stop Solution	11 ml
7. Wash Concentrate 20x: 1 Bottle	25 ml

Standard Concentrations and example data

	OD 450 nm	Conc. ng/mL
Std 1	0.045	0
Std 2	0.191	25
Std 3	0.628	100
Std 4	1.445	250
Std 5	2.178	500
Std 6	2.896	1000

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MB, ELISA Target remove.

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