Click image to see more details
Product Info Summary
| SKU: | EK2325 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, tissue, serum, plasma(heparin, EDTA) |
Product info
Product Name
Human Neuropeptide Y/NPY ELISA Kit PicoKine®
SKU/Catalog Number
EK2325
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Neuropeptide Y/NPY ELISA Kit PicoKine® (96 Tests). Quantitate Human Neuropeptide Y/NPY in cell culture supernatants, tissue, serum, plasma (heparin, EDTA). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Neuropeptide Y/NPY ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2325)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Y29-Y64
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<30 pg/mL
Assay Range
312.0-10,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2325 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2325 is reactive to NPY in Human samples
Validated Sample Types
cell culture supernatants, tissue, serum, plasma(heparin, EDTA)
Application Guarantee
EK2325 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NPY
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2325-CAP | Anti-Human NPY Pre-coated 96-well strip microplate | 1 |
| EK2325-ST | Human NPY Standard | 2 vials, 10 ng/tube |
| EK2325-DA | Human NPY Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | |
| O.D. | 0.051 | 0.074 | 0.119 | 0.23 | 0.47 | 1.049 | 1.968 | nan |
Data Example Images
Click image to see more details
Human Neuropeptide Y/NPY PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Neuropeptide Y/NPY ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 450 | 2022 | 6602 | 355 | 1400 | 7135 |
| Standard deviation | 18.23 | 75.83 | 270.68 | 12.60 | 91.84 | 487.32 |
| CV (%) | 4.1% | 3.8% | 4.1% | 3.6% | 6.6% | 6.8% |
Specific Publications For Human Neuropeptide Y/NPY ELISA Kit PicoKine® (EK2325)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
0 Reviews For Human Neuropeptide Y/NPY ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
