Human NT-4 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NT-4. 96wells/kit, with removable strips. Cited in 2 publication(s).
Human NT-4 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates and serum.|
|Sample Volume:||100μl per well|
AKR7A2 participates in the detoxification of aldehydes and ketones. AKR7A2 catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. AKR7A2 is involved in producing the neuromodulator gamma-hydroxybutyrate (GHB). AKR7A2 has extensive substrate specificity. AKR7A2 shows NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). AKR7A2 reduces 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). AKR7A2 reduces the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. AKR7A2 takes part in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
|Product Name||Human NT-4 ELISA Kit PicoKine™
See all NTF4 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Neurotrophin 4/NTF4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Neurotrophin 4/NTF4 tissue specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.|
|Cite This Product||Human NT-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0475)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates and serum.|
|Immunogen||Expression system for standard: sf21; Immunogen sequence: G81-A210|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Target-derived survival factor for peripheral sensory sympathetic neurons.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Human NT-4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0475-CAP||96-well plate precoated with anti-Human NTF4 antibody||1|
|EK0475-ST||lyophilized recombinant Human NTF4 standard||10ng/tube|
|EK0475-DA||biotinylated anti-Human NTF4 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0475 Human NT-4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human NT-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human NT-4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 2
|Sun Y, Zhang J, Li C, Wang D, Ma Y, Sun Y, Liu Z, Wang C, Zhou X. Theriogenology. 2012 Jul 1;78(1):39-48. Doi: 10.1016/J.Theriogenology.2012.01.016. Epub 2012 Mar 28. Interaction Between Neurotrophin 4 And Gonadotrophin In Bovine Oviducts.||PubMed ID 22460152|
|Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration||PubMed ID 27411313|
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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