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Product Info Summary
| SKU: | EK1849 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human Omentin/intelectin-1 ELISA Kit PicoKine®
View all Intelectin-1/Omentin ELISA kits
SKU/Catalog Number
EK1849
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Omentin/intelectin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ITLN1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Omentin/intelectin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1849)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: T19-S298
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1849 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1849 is reactive to Itln1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1849 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Intelectin-1/Omentin
Intelectin-1, also known as omentin or intestinal lactoferrin receptor, is an intelectin encoded in humans by the ITLN1 gene. It is mapped to 1q23.3. Intelectin-1 functions both as a receptor for bacterial arabinogalactans and for lactoferrin. Having conserved ligand binding site residues, both human and mouse intelectin-1 bind the exocyclic vicinal diol of carbohydrate ligands such as galactofuranose.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1849-CAP | Anti-Human Itln1 Pre-coated 96-well strip microplate | 1 |
| EK1849-ST | Human Itln1 Standard | 2 vials, 10 ng/tube |
| EK1849-DA | Human Itln1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.033 | 0.094 | 0.171 | 0.283 | 0.515 | 0.929 | 1.244 | 2.019 |
Data Example Images
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Human Omentin/intelectin-1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Intelectin-1/Omentin, For serum, dilution ratio is 1:1 and concentration is around 100 pg/ml.
For plasma, dilution ratio is 1:5 and concentration is around 2.5 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Omentin/intelectin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 95 | 293 | 1596 | 100 | 395 | 1791 |
| Standard deviation | 4.85 | 15.53 | 89.38 | 6.60 | 26.86 | 127.16 |
| CV (%) | 5.1% | 5.3% | 5.6% | 6.6% | 6.8% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Intelectin-1/Omentin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 95 | 112 | 103 | 111 | 105 | 6.86 | 6.5% |
| Sample 2 | 293 | 296 | 263 | 269 | 280 | 14.44 | 5.1% |
| Sample 3 | 1596 | 1398 | 1585 | 1542 | 1530 | 78.97 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For Itln1 (Source: Uniprot.Org, NCBI)
Gene Name
Itln1
Full Name
Intelectin-1a
Weight
34.953kDa
Alternative Names
Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1 Itln1|I, IntL, It, Itln, Itln2, Itln3, Itln5, Itlna, Lfr, mL|intelectin 1 (galactofuranose binding)|intelectin-1a|calcium-dependent galactosyl-specific lectin|galactofuranose-binding lectin|intelectin 2|intelectin 5|intelectin a|intestinal lactoferrin receptor|truncated intelectin 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Itln1, check out the Itln1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Itln1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Omentin/intelectin-1 ELISA Kit PicoKine® (EK1849)
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No publications found for EK1849
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human Omentin/intelectin-1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-07-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-09
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Intelectin-1 using the EK1849 Human Intelectin-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-07-26
Question
Q: how can I thaw whole blood sample for Intelectin-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-05-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Intelectin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-05-04
Question
Q: if the enzyme conjugated Intelectin-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Intelectin-1 antigen?
Verified Customer
Verified customer
Asked: 2019-04-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. White
Verified customer
Asked: 2019-03-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-03-24
Question
Q: Can Intelectin-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-10-09
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Intelectin-1 level in serum.
Verified Customer
Verified customer
Asked: 2015-10-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-10-18
Question
Q: can I use EDTA plasma as samples in Human Intelectin-1 Picokine® ELISA Kit (Catalog # EK1849)?
Verified Customer
Verified customer
Asked: 2014-04-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Intelectin-1 causing degradation of its protein structure. Intelectin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Intelectin-1 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-04-09
