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Product Info Summary
| SKU: | EK2270 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human OSCAR ELISA Kit PicoKine®
SKU/Catalog Number
EK2270
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human OSCAR ELISA Kit PicoKine® (96 Tests). Quantitate Human OSCAR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human OSCAR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2270)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-F219
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2270 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2270 is reactive to OSCAR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2270 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of OSCAR
Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2270-CAP | Anti-Human OSCAR Pre-coated 96-well strip microplate | 1 |
| EK2270-ST | Human OSCAR Standard | 2 vials, 10 ng/tube |
| EK2270-DA | Human OSCAR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.041 | 0.169 | 0.272 | 0.496 | 0.686 | 0.974 | 1.375 | 2.053 |
Data Example Images
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Human OSCAR PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human OSCAR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 233 | 463 | 4178 | 165 | 552 | 4061 |
| Standard deviation | 10.42 | 33.61 | 166.57 | 9.62 | 37.81 | 189.24 |
| CV(%) | 4.5% | 7.3% | 2.8% | 5.8% | 6.9% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of OSCAR in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 233 | 204 | 263 | 232 | 233 | 15.52 | 6.7% |
| Sample 2 | 463 | 406 | 625 | 332 | 457 | 10.09 | 2.2% |
| Sample 3 | 4178 | 2680 | 3831 | 3264 | 3488 | 223.25 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For OSCAR (Source: Uniprot.Org, NCBI)
Gene Name
OSCAR
Full Name
Osteoclast-associated immunoglobulin-like receptor
Weight
30.481kDa
Superfamily
leukocyte receptor complex/polymeric immunogobulin receptor (PIR/LRC) family
Alternative Names
hOSCAR; MGC33613; OSCAR; osteoclast associated receptor OSCAR-S1; osteoclast associated receptor OSCAR-S2; osteoclast associated, immunoglobulin-like receptor; osteoclast-associated immunoglobulin-like receptor; Osteoclast-associated receptor; PIGR3; PIgR-3; Poly-Ig receptor 3; Polymeric immunoglobulin receptor 3 OSCAR PIGR3, PIgR-3 osteoclast associated Ig-like receptor osteoclast-associated immunoglobulin-like receptor|osteoclast associated receptor OSCAR-S1|osteoclast associated receptor OSCAR-S2|osteoclast associated, immunoglobulin-like receptor|poly-Ig receptor 3|polymeric immunoglobulin receptor 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on OSCAR, check out the OSCAR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for OSCAR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human OSCAR ELISA Kit PicoKine® (EK2270)
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