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Product Info Summary
| SKU: | EK2282 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human OSMRb ELISA Kit PicoKine®
SKU/Catalog Number
EK2282
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human OSMRb ELISA Kit PicoKine®(96 Tests). Quantitate Human OSMR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human OSMRb ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2282)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E24-L738
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2282 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2282 is reactive to OSMR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2282 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of OSMR beta
Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2282-CAP | Anti-Human OSMR Pre-coated 96-well strip microplate | 1 |
| EK2282-ST | Human OSMR Standard | 2 vials, 10 ng/tube |
| EK2282-DA | Human OSMR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.027 | 0.169 | 0.271 | 0.499 | 0.654 | 0.988 | 1.369 | 2.055 |
Data Example Images
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Human OSMRb ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human OSMRb ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 307 | 496 | 3021 | 265 | 461 | 3015 |
| Standard deviation | 22.07 | 32.98 | 80.36 | 18.47 | 14.11 | 173.97 |
| CV(%) | 7.2% | 6.7% | 2.7% | 7% | 3.1% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of OSMR beta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 307 | 165 | 291 | 270 | 258 | 6.95 | 2.7% |
| Sample 2 | 496 | 458 | 396 | 543 | 473 | 22.86 | 4.8% |
| Sample 3 | 3021 | 2785 | 3127 | 3535 | 3117 | 237.2 | 7.6% |
Protein Target Info & Infographic
Gene/Protein Information For OSMR (Source: Uniprot.Org, NCBI)
Gene Name
OSMR
Full Name
Oncostatin-M-specific receptor subunit beta
Weight
110.509kDa
Superfamily
type I cytokine receptor family
Alternative Names
IL-31 receptor subunit beta; IL-31R subunit beta; IL-31RB; IL-31R-beta; Interleukin-31 receptor subunit beta; MGC150627; MGC75127; oncostatin M receptor; oncostatin-M specific receptor beta subunit; oncostatin-M-specific receptor subunit beta; OSM R beta; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1 OSMR IL-31R-beta, IL-31RBB, OSMRbeta, PLCA1, OSMR oncostatin M receptor oncostatin-M-specific receptor subunit beta|IL-31 receptor subunit beta|IL-31R subunit beta|interleukin-31 receptor subunit beta|oncostatin-M specific receptor beta subunit
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on OSMR, check out the OSMR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for OSMR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human OSMRb ELISA Kit PicoKine® (EK2282)
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