Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine®

Product Name

Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine®

SKU/Catalog Number

EK2017

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Osteoadherin ELISA Kit PicoKine® (96 Tests). Quantitate Human OMD in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2017)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q21-E421

Capture/Detection Antibodies

The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

See datasheet of EK2017 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2017 is reactive to OMD in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2017 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of OMD

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 9q22.31. Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20‑30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix. Human OSAD is expressed as an even larger 110 kDa molecule in teeth. OSAD contains eight sulfated tyrosine residues and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C‑terminal acidic domain. OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Kit Components

Catalog Number	Description	Quantity
EK2017-CAP	Anti-Human OMD Pre-coated 96-well strip microplate	1
EK2017-ST	Human OMD Standard	2 vials, 10 ng/tube
EK2017-DA	Human OMD Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.084	0.165	0.231	0.373	0.588	0.93	1.489	2.053

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect OMD, Dilution ratio of 1:10, concentration in serum and plasma is 3-6 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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