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Product Info Summary
| SKU: | EK1447 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PD-L2/B7-DC ELISA Kit PicoKine®
SKU/Catalog Number
EK1447
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PD-L2/B7-DC ELISA Kit PicoKine® (96 Tests). Quantitate Human PDCD1LG2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PD-L2/B7-DC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1447)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L20-T220
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1447 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1447 is reactive to PDCD1LG2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1447 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PDCD1LG2
Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1447-CAP | Anti-Human PDCD1LG2 Pre-coated 96-well strip microplate | 1 |
| EK1447-ST | Human PDCD1LG2 Standard | 2 vials, 10 ng/tube |
| EK1447-DA | Human PDCD1LG2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.055 | 0.106 | 0.161 | 0.258 | 0.448 | 0.797 | 1.365 | 2.007 |
Data Example Images
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Human PD-L2/B7-DC PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PD-L2/B7-DC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 133 | 396 | 998 | 133 | 415 | 903 |
| Standard deviation | 7.18 | 28.11 | 57.318 | 7.31 | 37.35 | 54.18 |
| CV (%) | 5.4% | 7.1% | 5.8% | 5.5% | 9% | 6% |
Specific Publications For Human PD-L2/B7-DC ELISA Kit PicoKine® (EK1447)
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Customer Reviews
0 Reviews For Human PD-L2/B7-DC ELISA Kit PicoKine®
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Human PD-L2/B7-DC ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for PD-L2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PD-L2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-11-13
Question
Q: if the enzyme conjugated PD-L2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PD-L2 antigen?
Verified Customer
Verified customer
Asked: 2020-06-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-14
Question
Q: can you recommend the dilution ratio of serum samples for detection of PD-L2 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
W. Evans
Verified customer
Asked: 2020-03-11
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PD-L2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-11
Question
we received this customer inquiry regarding the product listed above. Does the kit recognize sPDL2 (soluble PDL2)? Which region does the antibody of the elisa recognize?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
EK1447 recognizes soluble PDL2.
Boster Scientific Support
Answered: 2020-02-27
Question
Does EK1447 recognize soluble PDL2?
Verified customer
Asked: 2019-09-20
Answer
The Human PD-L2/B7-DC ELISA Kit PicoKine™ (EK1447) recognizes soluble PDL2. The immunogen sequence for the standard, detection antibody, and capture antibody is L20-T220 (uniprot: Q9BQ51).
Boster Scientific Support
Answered: 2019-09-22
Question
Does EK1447 recognize sPDL2 (soluble PDL2)?
Verified customer
Asked: 2019-09-19
Answer
Yes, the Human PD-L2/B7-DC ELISA Kit PicoKine™ (EK1447) recognizes soluble PDL2.
Boster Scientific Support
Answered: 2019-09-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Chakrabarti
Verified customer
Asked: 2019-08-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-12
Question
Q: how to proceed with the analysis of ELISA data? I measured PD-L2 level in serum.
V. Zhou
Verified customer
Asked: 2018-07-01
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-07-01
Question
Q: is it okay to use citrate plasma as samples in Human PD-L2 Picokine® ELISA Kit (Catalog # EK1447)?
W. Gandhi
Verified customer
Asked: 2016-07-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of PD-L2 causing degradation of its protein structure. PD-L2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the PD-L2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-07-02
