Human PDGF-AA ELISA Kit PicoKine™

Human PDGF-AA ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDGFA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .

Product Info Summary

SKU: EK1696
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human PDGF-AA ELISA Kit PicoKine™

See all PDGFR alpha products

SKU/Catalog Number

EK1696

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PDGF-AA ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDGFA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human PDGF-AA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1696)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S87-T211

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1696 is reactive to PDGFA in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1696 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1696-CAP Anti-Human PDGFA Pre-coated 96-well strip microplate 1
EK1696-ST Human PDGFA Standard 2 vials, 10 ng/tube
EK1696-DA Human PDGFA Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631362512502500500010000
O.D.0.1260.1980.2840.3610.5790.8621.2571.950

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect PDGFR alpha, Dilution ratio of 1:200, concentration in serum is 180-400ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PDGF-AA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2771292421429813034659
Standard deviation16.985.27227.5612.862.5349.4
CV (%)6.1%6.6%5.4%4.3%4.8%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PDGFR alpha in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127725625529026914.735.4%
Sample . 21292137712931260130543.363.3%%
Sample 342143960430239844115146.593.5%
*number of samples for each test n=16.

Gene/Protein Information For PDGFA (Source: Uniprot.Org, NCBI)

Uniprot ID

P16234

Gene ID

5156

Gene Name

PDGFA

Full Name

Platelet-derived growth factor receptor alpha

Weight

122.67kDa

Superfamily

protein kinase superfamily

Alternative Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA PDGFRA CD140A, PDGFR-2, PDGFR2 platelet derived growth factor receptor alpha platelet-derived growth factor receptor alpha|CD140 antigen-like family member A|CD140a antigen|PDGF-R-alpha|alpha-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 2|platelet-derived growth factor receptor, alpha polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PDGFA, check out the PDGFA Infographic

PDGFA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PDGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Human PDGF-AA ELISA Kit PicoKine™

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Pdgfa using the EK1696 Human Pdgfa Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-06-16

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-06-16

Question

Q: how do I analyze ELISA data? I have obtained Pdgfa level in serum.

Verified Customer

Verified customer

Asked: 2019-11-02

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-25

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

H. Adams

Verified customer

Asked: 2018-09-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-09-29

Question

Q: is it okay to use citrate plasma as samples in Human Pdgfa Picokine® ELISA Kit (Catalog # EK1696)?

Verified Customer

Verified customer

Asked: 2018-01-20

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Pdgfa causing degradation of its protein structure. Pdgfa may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Pdgfa ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-01-20

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-10-23

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-10-23

Question

Q: how to thaw whole blood sample for Pdgfa ELISA after freezing?

E. Martin

Verified customer

Asked: 2016-09-19

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Pdgfa for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-09-19

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Pdgfa in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2015-06-20

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Pdgfa ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-06-20

Question

Q: What is the optimal O.D. value for Pdgfa ELISA kit? I used your Pdgfa ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Pdgfa even though the O.D. values are not very high?

P. Allen

Verified customer

Asked: 2015-01-06

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Pdgfa ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of Pdgfa that can be declared/interpreted as positive by the above standard is 10pg/ml .

Boster Scientific Support

Answered: 2015-01-06

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