- Table of Content
Human PEDF/SerpinF1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 20pg/ml. Cited in 1 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Assay Range:||0.78 ng/ml - 50 ng/ml|
|Product Name||Human PEDF/SerpinF1 ELISA Kit PicoKine™
See more Serpin F1/PEDF products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human PEDF/SerpinF1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 20pg/ml.|
|Cite This Product||Human PEDF/SerpinF1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0896)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
|Assay Range||0.78 ng/ml - 50 ng/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: M1-P418|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||5176|
|Species Of This Entry||Human|
|Protein Name||Pigment epithelium-derived factor|
|Alternative Names||Serpin F1/PEDF|Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for SERPINF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0896-CAP||Anti-Human SERPINF1 Pre-coated 96-well strip microplate||1|
|EK0896-ST||Human SERPINF1 Standard||2 vials, 50 ng/tube|
|EK0896-DA||Human SERPINF1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0896 Human PEDF/SerpinF1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human PEDF/SerpinF1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human PEDF/SerpinF1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Polyethylene glycol-modified pigment epithelial-derived factor: new prospects for treatment of retinal neovascularization||PubMed ID 22495066|
Domestically in USA and Canada, ships next business day if available in stock.
Comprehensive info on SERPINF1, beautifully presented.Open Infographic
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
We promise all of our products perform as described in datasheets.