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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human Periostin/OSF2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Periostin/OSF2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human POSTN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Periostin/OSF2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0985)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: N22-Q836
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0985 is reactive to POSTN in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0985 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Periostin/OSF-2
Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
|EK0985-CAP||Anti-Human POSTN Pre-coated 96-well strip microplate||1|
|EK0985-ST||Human POSTN Standard||2 vials, 10 ng/tube|
|EK0985-DA||Human POSTN Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Periostin/OSF2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Periostin/OSF-2, Dilution ratio of 1:50, concentration in serum is around 60ng/ml.
Some articles we found to cite concentrations of Periostin/OSF-2 in samples: 19212687 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Periostin/OSF2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Periostin/OSF-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For POSTN (Source: Uniprot.Org, NCBI)
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 POSTN OSF-2, OSF2, PDLPOSTN, PN periostin periostin|osteoblast specific factor 2 (fasciclin I-like)|periodontal ligament-specific periostin|periostin, osteoblast specific factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on POSTN, check out the POSTN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for POSTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0985 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Circulating Levels of IL-13, TGF-β1, and Periostin as Potential Biomarker for Coronary Artery Disease with Acute Heart Failure
TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis
Dysregulation of astrocyte extracellular signaling in Costello syndrome
TGF?3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis
Li Y, Xie K, Chen H, Wang G, Yu Y. J Surg Res. 2015 Jun 1;196(1):136-48. Doi: 10.1016/J.Jss.2015.02.042. Epub 2015 Feb 27. Hydrogen Gas Inhibits High-Mobility Group Box 1 Release In Septic Mice By Upregulation Of Heme Oxygenase 1.
Krencik R, Hokanson Kc, Narayan Ar, Dvornik J, Rooney Ge, Rauen Ka, Weiss La, Rowitch Dh, Ullian Em. Sci Transl Med. 2015 May 6;7(286):286Ra66. Doi: 10.1126/Scitranslmed.Aaa5645. Dysregulation Of Astrocyte Extracellular Signaling In Costello Syndr...
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