Overview
| Product Name |
Human Periostin/OSF2 ELISA Kit PicoKine™
See all Periostin/OSF-2 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0985 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Periostin/POSTN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Periostin/POSTN tissue specificity: Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. . |
| Cite This Product |
Human Periostin/OSF2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0985)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
156pg/ml-10000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: N22-Q836 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For POSTN (Source: Uniprot.org, NCBI)
| Uniprot Id | Q15063 |
|---|
| NCBI Gene Id | 10631 |
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| Species Of This Entry | Human |
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| Gene Name | POSTN |
|---|
| Protein Name | Periostin |
|---|
| Alternative Names | Periostin/OSF-2|Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 |
|---|
| Molecular Weight | 93314 |
|---|
| Post Tranlational Modifications | Phosphorylation; Cleavage; Acetylation; Sulphation; Carboxylation; Reduction; Glycosylation; Gamma-carboxylation; Methylation |
|---|
| Subcellular Localization | Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi. |
|---|
| Gene Location | 13q13.3, on Chromasome 13, gene sequence: NC_000013.11 (37562582..37598844, complement) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For POSTN (Source: Uniprot.org, NCBI)
| Protein Function | Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). |
|---|
| Tissue Specificity | Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. |
|---|
| Related Diseases | Malignant Neoplasms; Neoplasms; Tissue Adhesions; Neoplasm Metastasis; Carcinoma; Cell Invasion; Fibrosis; Neoplasm Invasiveness; Periosteal Disorder; Inflammation; Tumor Angiogenesis; Asthma; Lung Neoplasms; Mammary Neoplasms; Malignant Neoplasm Of Breast; Malignant Paraganglionic Neoplasm; Hypertrophy; Heart Failure; Malignant Squamous Cell Neoplasm; Myocardial Infarction |
|---|
| Related Pathways | Cell Adhesion; Pathogenesis; Angiogenesis; Regeneration; Cell Proliferation; Localization; Cell Migration; Secretion; Osteoblast Differentiation; Cell Growth; Cell Cycle; Ossification; Heart Development; Bone Development; Tissue Regeneration; Tissue Development; Tooth Eruption; Cell Differentiation; Aging; Reverse Transcription |
|---|
| Background | Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis. |
|---|
| Scientific References | PMID: 8363580 by Takeshita S., et al. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I.
PMID: 12235007 by Gillan L., et al. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility. |
|---|
Kit Components And Assay QC Data Details Of Human Periostin/OSF2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0985-CAP | 96-well plate precoated with anti-Human Periostin/OSF-2 antibody | 1 |
| EK0985-ST | lyophilized recombinant Human Periostin/OSF-2 standard | 10ng/tube |
| EK0985-DA | biotinylated anti-Human Periostin/OSF-2 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0985 Human Periostin/OSF2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.010 | 0.100 | 0.166 | 0.302 | 0.582 | 0.978 | 1.604 | 2.279 |
We measured random samples of Human Periostin/OSF2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 188 | 1707 | 4824 | 180 | 1664 | 4867 |
| Standard deviation | 8.64 | 124.61 | 192.96 | 11.34 | 136.44 | 199.54 |
| CV(%) | 4.6% | 7.3% | 4% | 8.2% | 8.2% | 4.1% |
We measured the performance consistency of Human Periostin/OSF2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 188 | 183 | 213 | 194 | 194 | 11.36 | 5.8% |
| Sample 2 | 1707 | 1857 | 1800 | 1793 | 1789 | 53.58 | 2.9% |
| Sample 3 | 4824 | 4787 | 4733 | 4555 | 4724 | 103.2 | 2.1% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Periostin/OSF-2 primary antibodies,
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Total number of citations: 3
| Krencik R, Hokanson Kc, Narayan Ar, Dvornik J, Rooney Ge, Rauen Ka, Weiss La, Rowitch Dh, Ullian Em. Sci Transl Med. 2015 May 6;7(286):286Ra66. Doi: 10.1126/Scitranslmed.Aaa5645. Dysregulation Of Astrocyte Extracellular Signaling In Costello Syndr... |
PubMed ID 25947161 |
| Li Y, Xie K, Chen H, Wang G, Yu Y. J Surg Res. 2015 Jun 1;196(1):136-48. Doi: 10.1016/J.Jss.2015.02.042. Epub 2015 Feb 27. Hydrogen Gas Inhibits High-Mobility Group Box 1 Release In Septic Mice By Upregulation Of Heme Oxygenase 1. |
PubMed ID 25818978 |
| TGF?3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis |
PubMed ID 26857387 |
