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Product Info Summary
| SKU: | EK2326 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Human Plasminogen ELISA Kit PicoKine®
SKU/Catalog Number
EK2326
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Plasminogen ELISA Kit PicoKine® (96 Tests). Quantitate Human Plasminogen L2A1 in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Plasminogen ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2326)
Clonality of Antibodies
See Datasheet for details
Standard Protein
human brain
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<10 pg/mL
Assay Range
78.1-5,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2326 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2326 is reactive to PLG in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2326 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PLG
PLAUR (PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2326-CAP | Anti-Human PLG Pre-coated 96-well strip microplate | 1 |
| EK2326-ST | Human PLG Standard | 2 vials, 10 ng/tube |
| EK2326-DA | Human PLG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.009 | 0.077 | 0.156 | 0.257 | 0.448 | 0.761 | 1.188 | 2.012 |
Data Example Images
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Human Plasminogen PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Plasminogen ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 132 | 603 | 2362 | 96 | 603 | 1393 |
| Standard deviation | 7.68 | 32.74 | 170.77 | 5.62 | 15.86 | 57.67 |
| CV (%) | 5.8% | 5.4% | 7.2% | 5.9% | 2.6% | 4.1% |
Specific Publications For Human Plasminogen ELISA Kit PicoKine® (EK2326)
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