Human Plexin B2/PLXNB2 ELISA Kit PicoKine™

Human Plexin B2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1826
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human Plexin B2/PLXNB2 ELISA Kit PicoKine™

See all Plexin B2 products

SKU/Catalog Number

EK1826

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Plexin B2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Plexin B2/PLXNB2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1826)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L1838

Sensitivity

<0.1 ng/ml

Assay Range

0.624 ng/ml - 40 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1826 is reactive to PLXNB2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1826 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1826-CAP Anti-Human PLXNB2 Pre-coated 96-well strip microplate 1
EK1826-ST Human PLXNB2 Standard 2 vials, 40 ng/tube
EK1826-DA Human PLXNB2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.631.252.55102040
O.D.0.0290.1500.2990.3810.7310.9291.3461.963

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Plexin B2, Dilution ratio of 1:4, concentration in serum is around 20ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Plexin B2/PLXNB2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12144275196031233440719741
Standard deviation63.13239.4960.5582.6339.31539.8
CV (%)5.2%5.6%4.9%6.7%7.7%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Plexin B2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11214101811651017110387.727.9%
Sample 242754570451147414524166.863.6%
Sample 31960318628195711828319021578.863.0%
*number of samples for each test n=16.

Gene/Protein Information For PLXNB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15031

Gene ID

23654

Gene Name

PLXNB2

Full Name

Plexin-B2

Weight

205.127kDa

Superfamily

plexin family

Alternative Names

MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2 PLXNB2 MM1, Nbla00445, PLEXB2, dJ402G11.3 plexin B2 plexin-B2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLXNB2, check out the PLXNB2 Infographic

PLXNB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLXNB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1826

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5 Customer Q&As for Human Plexin B2/PLXNB2 ELISA Kit PicoKine™

Question

Q: how do I analyze ELISA data? I have performed Plexin B2 level in plasma.

Z. Dayal

Verified customer

Asked: 2020-05-13

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-05-13

Question

Q: if the enzyme conjugated Plexin B2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Plexin B2 antigen?

M. Chakrabarti

Verified customer

Asked: 2020-01-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-01-04

Question

Q: Can Plexin B2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

D. Singh

Verified customer

Asked: 2019-06-16

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-06-16

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-09-11

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-09-11

Question

Q: how to thaw whole blood sample for Plexin B2 ELISA after freezing?

L. Jackson

Verified customer

Asked: 2014-05-26

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Plexin B2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2014-05-26

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