Human PON1/Paraoxonase 1 ELISA Kit PicoKine™

Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PON1 in cell culture supernatants, cell lysates, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1141
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin).

Product Name

Human PON1/Paraoxonase 1 ELISA Kit PicoKine™

See all PON1 products

SKU/Catalog Number

EK1141

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PON1 in cell culture supernatants, cell lysates, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1141)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-L355

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1141 is reactive to PON1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin).

Application Guarantee

EK1141 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1141-CAP Anti-Human PON1 Pre-coated 96-well strip microplate 1
EK1141-ST Human PON1 Standard 2 vials, 2 ng/tube
EK1141-DA Human PON1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0530.0970.1560.2440.5851.0351.4792.036

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect PON1, Dilution ratio of 1:1000, concentration in serum is around 250ng/ml.

Some articles we found to cite concentrations of PON1 in samples: 25551104 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4920391747199834
Standard deviation2.1513.1942.182.1615.5240.86
CV (%)4.4%6.5%4.6%4.6%7.8%4.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PON1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 149524546482.735.6%
Sample 22032232262232189.174.2%
Sample 391792380690788847.825.3%
*number of samples for each test n=16.

Gene/Protein Information For PON1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P27169

Gene ID

5444

Gene Name

PON1

Full Name

Serum paraoxonase/arylesterase 1

Weight

39.731kDa

Superfamily

paraoxonase family

Alternative Names

A-esterase 1; Aromatic esterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; K-45; MVCD5; paraoxonase 1; PON 1; PON1; PONparaoxonase B-type; serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1 PON1 ESA, MVCD5, PON paraoxonase 1 serum paraoxonase/arylesterase 1|A-esterase 1|K-45|PON 1|aromatic esterase 1|arylesterase 1|arylesterase B-type|esterase A|paraoxonase B-type|serum aryldiakylphosphatase|serum aryldialkylphosphatase 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PON1, check out the PON1 Infographic

PON1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PON1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human PON1/Paraoxonase 1 ELISA Kit PicoKine™

Question

Do you have any assay data or results with human serum samples? Keyword: applications, reference

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

PON1 level in healthy human serum is non-detectable, it is below the lowest point on the standard curve.

Boster Scientific Support

Answered: 2020-04-01

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PON1 using the EK1141 Human PON1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-25

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-08-25

Question

What samples types have been tested with EK1141?

Verified customer

Asked: 2019-04-29

Answer

The Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ (EK1141) was also tested for cell culture supernates, cell lysates, and plasma(heparin) samples.

Boster Scientific Support

Answered: 2019-04-30

Question

Q: if the enzyme conjugated PON1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PON1 antigen?

L. Martin

Verified customer

Asked: 2018-11-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-11-14

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of PON1 in Human cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

S. Wilson

Verified customer

Asked: 2017-09-24

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PON1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-09-24

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

T. Jones

Verified customer

Asked: 2016-08-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-08-13

Question

Q: which procedure should I follow in order to thaw whole blood sample for PON1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-05-20

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PON1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-05-20

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