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Product Info Summary
| SKU: | EK1141 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin). |
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Product info
Product Name
Human PON1/Paraoxonase 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1141
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PON1 in cell culture supernatants, cell lysates, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PON1/Paraoxonase 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1141)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A2-L355
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1141 is reactive to PON1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin).
Application Guarantee
EK1141 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PON1
Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1141-CAP | Anti-Human PON1 Pre-coated 96-well strip microplate | 1 |
| EK1141-ST | Human PON1 Standard | 2 vials, 2 ng/tube |
| EK1141-DA | Human PON1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.053 | 0.097 | 0.156 | 0.244 | 0.585 | 1.035 | 1.479 | 2.036 |
Data Example Images
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Human PON1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PON1, Dilution ratio of 1:1000, concentration in serum is around 250ng/ml.
Some articles we found to cite concentrations of PON1 in samples: 25551104 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PON1/Paraoxonase 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 49 | 203 | 917 | 47 | 199 | 834 |
| Standard deviation | 2.15 | 13.19 | 42.18 | 2.16 | 15.52 | 40.86 |
| CV (%) | 4.4% | 6.5% | 4.6% | 4.6% | 7.8% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PON1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 49 | 52 | 45 | 46 | 48 | 2.73 | 5.6% |
| Sample 2 | 203 | 223 | 226 | 223 | 218 | 9.17 | 4.2% |
| Sample 3 | 917 | 923 | 806 | 907 | 888 | 47.82 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For PON1 (Source: Uniprot.Org, NCBI)
Uniprot ID
P27169
Gene ID
5444
Gene Name
PON1
Full Name
Serum paraoxonase/arylesterase 1
Weight
39.731kDa
Superfamily
paraoxonase family
Alternative Names
A-esterase 1; Aromatic esterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; K-45; MVCD5; paraoxonase 1; PON 1; PON1; PONparaoxonase B-type; serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1 PON1 ESA, MVCD5, PON paraoxonase 1 serum paraoxonase/arylesterase 1|A-esterase 1|K-45|PON 1|aromatic esterase 1|arylesterase 1|arylesterase B-type|esterase A|paraoxonase B-type|serum aryldiakylphosphatase|serum aryldialkylphosphatase 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PON1, check out the PON1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PON1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human PON1/Paraoxonase 1 ELISA Kit PicoKine® (EK1141)
EK1141 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pro-Oxidative and Proinflammatory Effects After Traveling From Los Angeles to Beijing
Species: Human
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Human PON1/Paraoxonase 1 ELISA Kit PicoKine®
Question
Do you have any assay data or results with human serum samples? Keyword: applications, reference
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
PON1 level in healthy human serum is non-detectable, it is below the lowest point on the standard curve.
Boster Scientific Support
Answered: 2020-04-01
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PON1 using the EK1141 Human PON1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-25
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-08-25
Question
What samples types have been tested with EK1141?
Verified customer
Asked: 2019-04-29
Answer
The Human PON1/Paraoxonase 1 ELISA Kit PicoKine™ (EK1141) was also tested for cell culture supernates, cell lysates, and plasma(heparin) samples.
Boster Scientific Support
Answered: 2019-04-30
Question
Q: if the enzyme conjugated PON1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PON1 antigen?
L. Martin
Verified customer
Asked: 2018-11-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-14
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of PON1 in Human cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
S. Wilson
Verified customer
Asked: 2017-09-24
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PON1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-09-24
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
T. Jones
Verified customer
Asked: 2016-08-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-08-13
Question
Q: which procedure should I follow in order to thaw whole blood sample for PON1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-05-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PON1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-05-20
