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Product Info Summary
| SKU: | EK2259 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human PREP ELISA Kit PicoKine®
View all Prolyl Oligopeptidase/PREP ELISA kits
SKU/Catalog Number
EK2259
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PREP ELISA Kit PicoKine® (96 Tests). Quantitate Human PREP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PREP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2259)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L2-P710
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2259 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2259 is reactive to PREP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2259 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Prolyl Oligopeptidase/PREP
Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene. The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2259-CAP | Anti-Human PREP Pre-coated 96-well strip microplate | 1 |
| EK2259-ST | Human PREP Standard | 2 vials, 10 ng/tube |
| EK2259-DA | Human PREP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.039 | 0.162 | 0.262 | 0.436 | 0.656 | 0.945 | 1.355 | 2.083 |
Data Example Images
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Human PREP PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PREP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 266 | 567 | 4581 | 259 | 412 | 4888 |
| Standard deviation | 18.97 | 26.76 | 349.53 | 8.52 | 14.05 | 147.62 |
| CV(%) | 7.1% | 4.7% | 7.6% | 3.3% | 3.4% | 3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Prolyl Oligopeptidase/PREP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 266 | 163 | 194 | 185 | 202 | 13.39 | 6.6% |
| Sample 2 | 567 | 457 | 337 | 594 | 489 | 20.19 | 4.1% |
| Sample 3 | 4581 | 3565 | 3862 | 4005 | 4003 | 136.51 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For PREP (Source: Uniprot.Org, NCBI)
Gene Name
PREP
Full Name
Prolyl endopeptidase
Weight
80.7kDa
Superfamily
peptidase S9A family
Alternative Names
dJ355L5.1 (prolyl endopeptidase); EC 3.4.21.26; MGC16060; PE; PEP; Post-proline cleaving enzyme; PREP; prolyl endopeptidase; Prolyl Oligopeptidase; rPop PREP PE, PEP prolyl endopeptidase prolyl endopeptidase|dJ355L5.1 (prolyl endopeptidase)|post-proline cleaving enzyme|prolyl oligopeptidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PREP, check out the PREP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PREP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PREP ELISA Kit PicoKine® (EK2259)
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