Human Pro-Cathepsin B ELISA Kit PicoKine®

Product Name

Human Pro-Cathepsin B ELISA Kit PicoKine®

View all Cathepsin B ELISA kits

SKU/Catalog Number

EK2130

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Pro-Cathepsin B ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSB in cell culture supernatants, serum, plasma (heparin), saliva and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Pro-Cathepsin B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2130)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: R18-I339

Sensitivity

<20 pg/ml

Assay Range

0.156 ng/ml - 10 ng/ml

Standard Dilution Instructions

See datasheet of EK2130 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2130 is reactive to CTSB in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin), saliva and urine

Application Guarantee

EK2130 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cathepsin B

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Kit Components

Catalog Number	Description	Quantity
EK2130-CAP	Anti-Human CTSB Pre-coated 96-well strip microplate	1
EK2130-ST	Human CTSB Standard	2 vials, 10 ng/tube
EK2130-DA	Human CTSB Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	0.156	0.312	0.625	1.25	2.5	5	10
O.D.	0.019	0.138	0.241	0.334	0.409	0.719	1.304	1.902

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Pro-Cathepsin B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cathepsin B in ELISA kits from four different production batches/lots.

Gene/Protein Information For CTSB (Source: Uniprot.Org, NCBI)

Alternative Names

CTSB; CPSB; Cathepsin B; EC 3.4.22.1; APP secretase; APPS; Cathepsin B1 [Cleaved into: Cathepsin B light chain; Cathepsin B heavy chain] CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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