Human Progesterone(P) ELISA Kit (Competitive ELISA)

ELISA kit

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Progesterone (P). 96 wells/kit. Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK7004
Size: 96 wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum

Product Name

Human Progesterone(P) ELISA Kit (Competitive ELISA)

SKU/Catalog Number

EK7004

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Progesterone (P). 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Progesterone(P) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7004)

Clonality of Antibodies

See Datasheet for details

Sensitivity

0.2 ng/ml

Assay Range

1-70 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7004 is reactive to in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7004 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Pre-coated 96-well strip microplate18 strips of 12 wellsGoat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate
Human Progesterone (P) Standards (S0~S5)60.5mlProgesterone (P) (0, 1, 3, 10, 30, 70 ng/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300
HRP Conjugated antigen16mlHRP Conjugated Human Progesterone (P), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300
Antibody16mlRabbit anti- Human Progesterone (P) Monoclonal Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue
Controls20.5mlProgesterone (P), 100% natural protein, 0.1% Proclin-300
Range of Control 1: 1.8-4.2 ng/mL
Range of Control 2: 18-42 ng/mL
20X Wash Buffer Concentrate115ml0.2M PBS containing 0.5% tween 20
Color Developing Reagent A17ml11m mol/L Urea hydrogen peroxide
Color Developing Reagent B17ml2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution17ml2mol/L Sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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1 Reviews For Human Progesterone(P) ELISA Kit (Competitive ELISA)

0

Superb Human Progesterone ELISA Kit From Boster

Excellent

Source: Biocompare.com

Application Human Progesterone saliva levels
Starting Material Saliva
Tips This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop.
Results Summary This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity.

"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."

Verified customer

Submitted

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