Product Info Summary
SKU: | EK7004 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
Product info
Product Name
Human Progesterone(P) ELISA Kit (Competitive ELISA)
SKU/Catalog Number
EK7004
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Progesterone (P). 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Progesterone(P) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7004)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.2 ng/ml
Assay Range
1-70 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7004 is reactive to in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7004 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Goat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate |
Human Progesterone (P) Standards (S0~S5) | 6 | 0.5ml | Progesterone (P) (0, 1, 3, 10, 30, 70 ng/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300 |
HRP Conjugated antigen | 1 | 6ml | HRP Conjugated Human Progesterone (P), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300 |
Antibody | 1 | 6ml | Rabbit anti- Human Progesterone (P) Monoclonal Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue |
Controls | 2 | 0.5ml | Progesterone (P), 100% natural protein, 0.1% Proclin-300 Range of Control 1: 1.8-4.2 ng/mL Range of Control 2: 18-42 ng/mL |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Progesterone(P) ELISA Kit standard curve
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Specific Publications For Human Progesterone(P) ELISA Kit (Competitive ELISA) (EK7004)
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Customer Reviews
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1 Reviews For Human Progesterone(P) ELISA Kit (Competitive ELISA)
0
Superb Human Progesterone ELISA Kit From Boster
Excellent
Source: Biocompare.com
Application | Human Progesterone saliva levels |
---|---|
Starting Material | Saliva |
Tips | This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop. |
Results Summary | This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity. |
"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."
Madison Schwartz
Verified customer
Submitted 2024-07-15
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