Featured Product
Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human RBP4/Retinol Binding Protein 4 PicoKine™ Fast ELISA Kit and many others.
Overview
| Product Name |
Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™
See all RBP4/Retinol-Binding Protein 4 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0831 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Retinol-Binding Protein 4/RBP4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin , EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Retinol-Binding Protein 4/RBP4 tissue specificity: |
| Cite This Product |
Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0831)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, serum, plasma(heparin , EDTA) and urine. |
| Sensitivity |
<10pg/ml |
| Assay Range |
312pg/ml-20000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: E19-L201 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For RBP4 (Source: Uniprot.org, NCBI)
| Uniprot Id | P02753 |
|---|
| NCBI Gene Id | 5950 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | RBP4 |
|---|
| Protein Name | Retinol-binding protein 4 |
|---|
| Superfamily | calycin superfamily |
|---|
| Alternative Names | RBP4/Retinol-Binding Protein 4|interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma |
|---|
| Molecular Weight | 23010 |
|---|
| Post Tranlational Modifications | Oxidation; Phosphorylation; Cleavage; Methylation; Demethylation; Reduction; Glycosylation; Acylation |
|---|
| Subcellular Localization | Secreted. |
|---|
| Gene Location | 10q23.33, on Chromasome 10, gene sequence: NC_000010.11 (93591694..93601744, complement) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For RBP4 (Source: Uniprot.org, NCBI)
| Protein Function | Retinol-binding protein that mediates retinol transport in blood plasma (PubMed:5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed:22665496). |
|---|
| Tissue Specificity | Detected in blood plasma and in urine (at protein level). |
|---|
| Interacting Proteins | TTR |
|---|
| Related Diseases | Insulin Resistance; Obesity; Diabetes Mellitus; Diabetes Mellitus, Non-insulin-dependent; Insulin Sensitivity; Inflammation; Metabolic Syndrome X; Impaired Glucose Tolerance; Fatty Liver; Cardiovascular Diseases; Liver Diseases; Hypertensive Disease; Steatosis; Dyslipidemias; Kidney Diseases; Steatohepatitis; Polycystic Ovary Syndrome; Atherosclerosis; Nonalcoholic Fatty Liver Disease |
|---|
| Related Pathways | Pathogenesis; Secretion; Transport; Glomerular Filtration; Insulin Secretion; Glucose Homeostasis; Excretion; Immune Response; Energy Homeostasis; Aging; Retinol Transport; Glucose Transport; Localization; Fatty Acid Oxidation; Cell Adhesion; Cell Differentiation; Parturition; Ovulation; Regulation Of Glucose Homeostasis; Coagulation |
|---|
| Background | Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda. |
|---|
| Scientific References | PMID: 6316270 by Colantuoni V., et al. Cloning and sequencing of a full length cDNA coding for human retinol-binding protein.
PMID: 2998779 by D'Onofrio C., et al. Structure and cell-specific expression of a cloned human retinol binding protein gene: the 5'-flanking region contains hepatoma specific transcriptional signals. |
|---|
Kit Components And Assay QC Data Details Of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0831-CAP | 96-well plate precoated with anti-Human RBP4/Retinol-Binding Protein 4 antibody | 1 |
| EK0831-ST | lyophilized recombinant Human RBP4/Retinol-Binding Protein 4 standard | 20ng/tube |
| EK0831-DA | biotinylated anti-Human RBP4/Retinol-Binding Protein 4 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0831 Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.029 | 0.066 | 0.108 | 0.215 | 0.495 | 1.074 | 1.866 | 2.408 |
We measured random samples of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 621 | 2874 | 9419 | 643 | 2778 | 10172 |
| Standard deviation | 44.09 | 227.04 | 668.74 | 53.36 | 247.24 | 752.72 |
| CV(%) | 7.1% | 7.9% | 7.1% | 8.3% | 8.9% | 7.4% |
We measured the performance consistency of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 621 | 574 | 637 | 603 | 608 | 23.39 | 3.8% |
| Sample 2 | 2874 | 2703 | 2656 | 2896 | 2782 | 104.37 | 3.7% |
| Sample 3 | 9419 | 8739 | 8492 | 8429 | 8769 | 392.34 | 4.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all RBP4/Retinol-Binding Protein 4 primary antibodies,
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
| Ding N, Luo S, Yu J, Zhou Y, Wu Z. Mol Vis. 2018 Mar 26;24:252-260. eCollection 2018. Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment |
PubMed ID 29618920 |
| Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients |
PubMed ID 27114982 |
| The effect of acitretin treatment on insulin resistance, retinol-binding protein-4, leptin, and adiponectin in psoriasis vulgaris: a noncontrolled study. |
PubMed ID 24021889 |
