Featured Product
Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human RBP4/Retinol Binding Protein 4 PicoKine™ Fast ELISA Kit and many others.
Overview
| Product Name |
Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™
See more RBP4/Retinol-Binding Protein 4 products |
| Catalog# |
EK0831 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.. Sensitivity: 10pg/ml. |
| Cite This Product |
Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0831)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
| Sensitivity |
<10 pg/ml |
| Assay Range |
312 pg/ml - 20,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: E19-L201 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For RBP4 (Source: Uniprot.org, NCBI)
| Uniprot Id | P02753 |
|---|
| NCBI Gene Id | 5950 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | RBP4 |
|---|
| Protein Name | Retinol-binding protein 4 |
|---|
| Superfamily | calycin superfamily |
|---|
| Alternative Names | RBP4/Retinol-Binding Protein 4|interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma |
|---|
| Molecular Weight | 23010 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RBP4, check out the RBP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for RBP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the RBP4 infographic
Kit Components And Assay QC Data Details Of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0831-CAP |
Anti-Human RBP4 Pre-coated 96-well strip microplate | 1 |
| EK0831-ST | Human RBP4 Standard | 2 vials, 20 ng/tube |
| EK0831-DA | Human RBP4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0831 Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.029 | 0.066 | 0.108 | 0.215 | 0.495 | 1.074 | 1.866 | 2.408 |
We measured random samples of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 621 | 2874 | 9419 | 643 | 2778 | 10172 |
| Standard deviation | 44.09 | 227.04 | 668.74 | 53.36 | 247.24 | 752.72 |
| CV (%) | 7.1% | 7.9% | 7.1% | 8.3% | 8.9% | 7.4% |
We measured the performance consistency of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 621 | 574 | 637 | 603 | 608 | 23.39 | 3.8% |
| Sample 2 | 2874 | 2703 | 2656 | 2896 | 2782 | 104.37 | 3.7% |
| Sample 3 | 9419 | 8739 | 8492 | 8429 | 8769 | 392.34 | 4.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 2
| Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients |
PubMed ID 27114982 |
| Ding N, Luo S, Yu J, Zhou Y, Wu Z. Mol Vis. 2018 Mar 26;24:252-260. eCollection 2018. Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment |
PubMed ID 29618920 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0831 Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™
7 Related Questions
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
S. Maharaj
Asked: 2020-09-12
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-09-12
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis RBP4 using the EK0831 Human RBP4 Picokine® ELISA Kit?
A. Surve
Asked: 2020-07-31
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-07-31
Question
Q: how to proceed with the analysis of ELISA data? I have performed RBP4 level in urine.
Verified Customer
Asked: 2020-02-08
Boster Scientific Support
Answered: 2020-02-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2019-09-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-28
Question
Q: which procedure should I follow in order to thaw whole blood sample for RBP4 ELISA after freezing?
K. Williams
Asked: 2018-09-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test RBP4 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-09-04
Question
Q: can I use citrate plasma as samples in Human RBP4 Picokine® ELISA Kit (Catalog # EK0831)?
Verified Customer
Asked: 2017-11-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of RBP4 causing degradation of its protein structure. RBP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the RBP4 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-11-13
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of RBP4 in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
K. Anand
Asked: 2016-03-27
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the RBP4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-03-27
