Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0831
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

See all RBP4/Retinol-Binding Protein 4 products

SKU/Catalog Number

EK0831

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0831)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-L201

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0831 is reactive to RBP4 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0831 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0831-CAP Anti-Human RBP4 Pre-coated 96-well strip microplate 1
EK0831-ST Human RBP4 Standard 2 vials, 20 ng/tube
EK0831-DA Human RBP4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0290.0660.1080.2150.4951.0741.8662.408

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect RBP4/Retinol-Binding Protein 4, Dilution ratio of 1:20000, concentration in serum is around 28ug/ml.

Some articles we found to cite concentrations of RBP4/Retinol-Binding Protein 4 in samples: 23423766 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)62128749419643277810172
Standard deviation44.09227.04668.7453.36247.24752.72
CV (%)7.1%7.9%7.1%8.3%8.9%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of RBP4/Retinol-Binding Protein 4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 162157463760360823.393.8%
Sample 228742703265628962782104.373.7%
Sample 394198739849284298769392.344.4%
*number of samples for each test n=16.

Gene/Protein Information For RBP4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02753

Gene ID

5950

Gene Name

RBP4

Full Name

Retinol-binding protein 4

Weight

23.01kDa

Superfamily

calycin superfamily

Alternative Names

interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma RBP4 MCOPCB10, RDCCAS retinol binding protein 4 retinol-binding protein 4|PRBP|RBP|plasma retinol-binding protein|retinol binding protein 4, plasma|retinol-binding protein 4, interstitial

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RBP4, check out the RBP4 Infographic

RBP4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RBP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0831 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients

Ding N, Luo S, Yu J, Zhou Y, Wu Z. Mol Vis. 2018 Mar 26;24:252-260. eCollection 2018. Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment

Have you used Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Human RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

S. Maharaj

Verified customer

Asked: 2020-09-12

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-12

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis RBP4 using the EK0831 Human RBP4 Picokine® ELISA Kit?

A. Surve

Verified customer

Asked: 2020-07-31

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-07-31

Question

Q: how to proceed with the analysis of ELISA data? I have performed RBP4 level in urine.

Verified Customer

Verified customer

Asked: 2020-02-08

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-08

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-09-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-09-28

Question

Q: which procedure should I follow in order to thaw whole blood sample for RBP4 ELISA after freezing?

K. Williams

Verified customer

Asked: 2018-09-04

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test RBP4 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-09-04

Question

Q: can I use citrate plasma as samples in Human RBP4 Picokine® ELISA Kit (Catalog # EK0831)?

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of RBP4 causing degradation of its protein structure. RBP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the RBP4 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-11-13

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of RBP4 in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

K. Anand

Verified customer

Asked: 2016-03-27

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the RBP4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-03-27

$400

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0831
$400.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.