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Product Info Summary
| SKU: | PROTAAQ91388 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant APRIL (A proliferation-inducing ligand) protein, AF
View all APRIL recombinant proteins
SKU/Catalog Number
PROTAAQ91388
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
APRIL is a member of tumor necrosis factor ligand superfamily that expressed by stromal tissue, macrophages, and T cells. APRIL is a 27.4 kDa protein containing 250 residues, which plays a critical role in modulating tumor progression. Besides, APRIL has been demonstrated to involve in regulating B and T cell survival, proliferation and differentiation via binding with TNFRSF13B/TACI and TNFRSF17/BCMA.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant APRIL (A proliferation-inducing ligand) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTAAQ91388)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 0.1% sarkosyl in 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Molecular weight
The protein has a calculated MW of 17.29 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measured by its ability to induce cell death in Jurkat cells. The ED₅₀ for this effect is 2.6-4.0 μg/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MAVLTQKQKKQHSVLHLVPINATSKDDSDVTEVMWQPALRRGRGLQAQGYGVRIQDAGVYLLYSQVLFQDVTFTMGQVVSREGQGRQETLFRCIRSMPSHPDRAYNSCYSAGVFHLHQGDILSVIIPRARAKLNLSPHGTFLGFVKL with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human APRIL
Specific Publications For Human recombinant APRIL (A proliferation-inducing ligand) protein, AF (PROTAAQ91388)
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