Product Info Summary
| SKU: | PROTP31371-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant FGF-9 (Fibroblast growth factor-9) protein, AF
View all FGF9 recombinant proteins
SKU/Catalog Number
PROTP31371-2
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Fibroblast Growth Factors-9 (FGF-9) is a 23.4 kDa member of the fibroblast Growth Factors with 208 amino acid residues. FGF-9 is an important role embryonic development, cell proliferation, cell differentiation and cell migration in cell functions. It can regulate bone development, glial cell growth and differentiation during development, angiogenesis, differentiation and survival of neuronal cells.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant FGF-9 (Fibroblast growth factor-9) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP31371-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
23.4 kDa
Molecular weight
The protein has a calculated MW of 22.14 kDa. The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
SDS- PAGE analysis of recombinant human FGF-9
Specific Publications For Human recombinant FGF-9 (Fibroblast growth factor-9) protein, AF (PROTP31371-2)
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