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Product Info Summary
| SKU: | PROTP14210-8 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | HEK293 cell |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant HGF protein, AF
View all HGF recombinant proteins
SKU/Catalog Number
PROTP14210-8
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Hepatocyte Growth Factor (HGF) activates c-Met receptor's tyrosine kinase cascade, influencing cell growth and motility. Originating from mesenchymal cells, HGF is a multifunctional cytokine that plays a central role in angiogenesis, tumorigenesis, and tissue regeneration. Initially secreted as an inactive polypeptide, HGF undergoes cleavage to become an active heterodimeric molecule. The gene exhibits alternative splicing, resulting in diverse isoforms.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant HGF protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP14210-8)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>90% as determined by SDS-PAGE.
Predicted MW
83.1 kDa
Molecular weight
The protein has a calculated MW of 80.48 kDa. The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
A DNA sequence encoding Human HGF Protein (#P14210)(Gln 32-Ser 728) was expressed with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human HGF
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