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Product Info Summary
| SKU: | PROTQ16270-4 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | HEK293 cell |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant IGFBP7(IGF-binding protein 7) protein, AF
View all IGFBP7 recombinant proteins
SKU/Catalog Number
PROTQ16270-4
Size
5ug,20ug,100ug
Tag
His-SUMO Tag (N-term)
Description
IGFBP7, also known as mac25, prostacyclin-stimulating factor (PSF), tumor adhesion factor (TAF), and angiomodulin (AGM), is a secreted protein that belongs to the insulin-like growth factor (IGF)-binding protein (IGFBP) family. In contrast to the other family members, IGFBP7 binds IGFs through the N-terminal domain with low affinity. IGFBP7 is expressed in various human tissues, including the brain, liver, pancreas, and skeletal muscle, and is secreted into circulation. IGF-BP7 modulates many biological functions, such as protein synthesis, proliferation, anti-apoptosis, and cell survival. Moreover, it has been reported that IGFBP7 promotes cardiac senescence through IGF-1R/IRS/AKT-dependent suppression of FOXO3a, inhibiting DNA repair, subsequently the leading to the progression of Heart failure. Human and mouse IGFBP7 are highly homologous, sharing 94% a.a. sequence identity.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant IGFBP7(IGF-binding protein 7) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ16270-4)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
29.1 kDa
Molecular weight
The protein has a calculated MW of 38.18 kDa. The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
A DNA sequence encoding Human IGFBP7 (#Q16270) (Ser27 – Leu282) was expressed with polyhistidine-SUMO tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human IGFBP7
Specific Publications For Human recombinant IGFBP7(IGF-binding protein 7) protein, AF (PROTQ16270-4)
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