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Product Info Summary
| SKU: | PROTP60568-7 |
|---|---|
| Size: | 100ug,1mg |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture, ELISA |
Product info
Product Name
Human recombinant IL-2 protein, GMP
View all IL2 recombinant proteins
SKU/Catalog Number
PROTP60568-7
Size
100ug,1mg
Tag
His Tag (C-term)
Description
Interleukin-2 (IL-2) is a 15.5 kDa four bundle, immuno-modulatory cytokine which is expressed predominately by antigen-simulated CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells (DC). IL-2 plays a fundamental role in promoting NK cell proliferation, cytotoxicity, and B cells differentiation. IL-2 modulate T cell differentiation programs, promoting na?ve CD4+ T cell differentiation into T helper-1 (Th1) and T helper-2 (Th2) cells.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant IL-2 protein, GMP (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP60568-7)
Form
Lyophilized
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Purity
>98% as determined by SDS-PAGE analysis.
Predicted MW
17.6 kDa
Molecular weight
The protein has a calculated MW of 16.4 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is <3 ng/mL. The specific activity of recombinant human IL-2 is >5 x 10⁶ IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).
Endotoxin
<0.05 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 min to ensure sufficient re-dissolved.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of GMP human IL-2
Specific Publications For Human recombinant IL-2 protein, GMP (PROTP60568-7)
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