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Product Info Summary
| SKU: | PROTQ2MKA7-1 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | HEK293 cell |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant R-spondin 1 protein, AF
View all RSPO1 recombinant proteins
SKU/Catalog Number
PROTQ2MKA7-1
Size
5ug,20ug,100ug
Tag
His-SUMO Tag (N-term)
Description
R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
Storage & Handling
Store at 2°C to 8°C for up to 2 weeks, -20°C or -80°C for 6 months. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant R-spondin 1 protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ2MKA7-1)
Form
Liquid
Formulation
Liquid (1X PBS, pH 7.4) If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
29.0 kDa
Molecular weight
The protein has a calculated MW of 46.8 kDa. The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).
Activity
R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is 1-3 μg/mL.
Endotoxin
<1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human R-spondin 1
Specific Publications For Human recombinant R-spondin 1 protein, AF (PROTQ2MKA7-1)
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