Human REG1A ELISA Kit PicoKine™

Human REG1A ELISA Kit PicoKine™ (96 Tests). Quantitate Human REG1A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1812
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human REG1A ELISA Kit PicoKine™

See all REG1A products

SKU/Catalog Number

EK1812

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human REG1A ELISA Kit PicoKine™ (96 Tests). Quantitate Human REG1A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human REG1A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1812)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-N166

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1812 is reactive to REG1A in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1812 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1812-CAP Anti-Human REG1A Pre-coated 96-well strip microplate 1
EK1812-ST Human REG1A Standard 2 vials, 10 ng/tube
EK1812-DA Human REG1A Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.1360.1510.1930.2810.4090.6911.3442.338

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect REG1A, Dilution ratio of 1:100, concentration in serum is around 60ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human REG1A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8978611691218451307
Standard deviation4.6351.0966.638.3549.0182.34
CV (%)5.2%6.5%5.7%6.9%5.8%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of REG1A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 187919187892.002.2%
Sample 279083178174578630.593.8%
Sample 31112125412211089116969.965.9%
*number of samples for each test n=16.

Gene/Protein Information For REG1A (Source: Uniprot.Org, NCBI)

Uniprot ID

P05451

Gene ID

5967

Gene Name

REG1A

Full Name

Lithostathine-1-alpha

Weight

18.731kDa

Alternative Names

ICRF; Islet cells regeneration factor; Islet of Langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; Pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; Reg1A; REG-1-alpha; Regenerating islet-derived protein 1-alpha; Regenerating protein I alpha REG1A ICRF, P19, PSP, PSPS, PSPS1, PTP, REG regenerating family member 1 alpha lithostathine-1-alpha|REG-1-alpha|islet cells regeneration factor|islet of langerhans regenerating protein|pancreatic stone protein, secretory|pancreatic thread protein|protein-X|regenerating islet-derived protein 1-alpha|regenerating protein I alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on REG1A, check out the REG1A Infographic

REG1A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for REG1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1812

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6 Customer Q&As for Human REG1A ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human REG1A Picokine® ELISA Kit (Catalog # EK1812)?

V. Wu

Verified customer

Asked: 2020-07-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of REG1A causing degradation of its protein structure. REG1A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the REG1A ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-07-08

Question

Q: how do I analyze ELISA data? I have performed REG1A level in plasma.

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-05-04

Question

Q: if the enzyme conjugated REG1A antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the REG1A antigen?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-04-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-11-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-11-17

Question

Q: Can REG1A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-06-14

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-06-14

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-01-17

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-01-17

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