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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human REG1A ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human REG1A ELISA Kit PicoKine™ (96 Tests). Quantitate Human REG1A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human REG1A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1812)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q23-N166
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1812 is reactive to REG1A in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK1812 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of REG1A
This gene is a type I subclass member of the Reg gene family. It is mapped to 2p12. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication.
|EK1812-CAP||Anti-Human REG1A Pre-coated 96-well strip microplate||1|
|EK1812-ST||Human REG1A Standard||2 vials, 10 ng/tube|
|EK1812-DA||Human REG1A Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human REG1A PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect REG1A, Dilution ratio of 1:100, concentration in serum is around 60ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human REG1A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of REG1A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For REG1A (Source: Uniprot.Org, NCBI)
ICRF; Islet cells regeneration factor; Islet of Langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; Pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; Reg1A; REG-1-alpha; Regenerating islet-derived protein 1-alpha; Regenerating protein I alpha REG1A ICRF, P19, PSP, PSPS, PSPS1, PTP, REG regenerating family member 1 alpha lithostathine-1-alpha|REG-1-alpha|islet cells regeneration factor|islet of langerhans regenerating protein|pancreatic stone protein, secretory|pancreatic thread protein|protein-X|regenerating islet-derived protein 1-alpha|regenerating protein I alpha*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on REG1A, check out the REG1A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for REG1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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