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Product Info Summary
| SKU: | EK1812 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human REG1A ELISA Kit PicoKine®
SKU/Catalog Number
EK1812
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human REG1A ELISA Kit PicoKine® (96 Tests). Quantitate Human REG1A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human REG1A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1812)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q23-N166
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1812 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1812 is reactive to REG1A in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1812 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of REG1A
This gene is a type I subclass member of the Reg gene family. It is mapped to 2p12. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1812-CAP | Anti-Human REG1A Pre-coated 96-well strip microplate | 1 |
| EK1812-ST | Human REG1A Standard | 2 vials, 10 ng/tube |
| EK1812-DA | Human REG1A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.044 | 0.102 | 0.156 | 0.293 | 0.532 | 0.938 | 1.577 | 2.278 |
Data Example Images
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Human REG1A PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect REG1A, Dilution ratio of 1:100, concentration in serum and plasma is around 60 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human REG1A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 89 | 786 | 1169 | 121 | 845 | 1307 |
| Standard deviation | 4.63 | 51.09 | 66.63 | 8.35 | 49.01 | 82.34 |
| CV (%) | 5.2% | 6.5% | 5.7% | 6.9% | 5.8% | 6.3% |
Specific Publications For Human REG1A ELISA Kit PicoKine® (EK1812)
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Recommended Resources
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Customer Reviews
0 Reviews For Human REG1A ELISA Kit PicoKine®
Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Human REG1A ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human REG1A Picokine® ELISA Kit (Catalog # EK1812)?
V. Wu
Verified customer
Asked: 2020-07-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of REG1A causing degradation of its protein structure. REG1A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the REG1A ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-08
Question
Q: how do I analyze ELISA data? I have performed REG1A level in plasma.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-05-04
Question
Q: if the enzyme conjugated REG1A antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the REG1A antigen?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-11-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-11-17
Question
Q: Can REG1A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-01-17
