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Product Info Summary
| SKU: | EK1249 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Renin / Prorenin ELISA Kit PicoKine®
SKU/Catalog Number
EK1249
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Renin / Prorenin ELISA Kit PicoKine™ (96 Tests). Quantitate Human REN in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Renin / Prorenin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1249)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L67-R406
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1249 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1249 is reactive to REN in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1249 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Renin
Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin system (RAS). This gene is mapped to 1q32.1. Renin's primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. What's more, Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1249-CAP | Anti-Human REN Pre-coated 96-well strip microplate | 1 |
| EK1249-ST | Human REN Standard | 2 vials, 10 ng/tube |
| EK1249-DA | Human REN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.034 | 0.063 | 0.087 | 0.124 | 0.304 | 0.530 | 1.145 | 2.113 |
Data Example Images
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Human Renin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Renin, Dilution ratio of 1:1, concentration in serum and plasma is 185-2270 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Renin / Prorenin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 73 | 224 | 875 | 68 | 211 | 909 |
| Standard deviation | 4.52 | 13.66 | 56 | 4.69 | 16.88 | 71.81 |
| CV (%) | 6.2% | 6.1% | 6.4% | 6.9% | 8% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Renin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 73 | 79 | 69 | 73 | 73 | 3.57 | 4.8% |
| Sample 2 | 224 | 224 | 208 | 232 | 222 | 8.71 | 3.9% |
| Sample 3 | 875 | 929 | 953 | 900 | 914 | 29.42 | 3.2% |
Protein Target Info & Infographic
Gene/Protein Information For REN (Source: Uniprot.Org, NCBI)
Gene Name
REN
Full Name
Renin
Weight
45.057kDa
Superfamily
peptidase A1 family
Alternative Names
angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; REN; renin precursor, renal; Renin REN ADTKD4, HNFJ2, RTD renin renin|angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on REN, check out the REN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for REN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Renin / Prorenin ELISA Kit PicoKine® (EK1249)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Human Renin / Prorenin ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Renin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Renin antigen?
Verified Customer
Verified customer
Asked: 2020-05-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Renin using the EK1249 Human Renin Picokine® ELISA Kit?
M. Bhasin
Verified customer
Asked: 2019-10-31
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-10-31
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
J. Turner
Verified customer
Asked: 2018-07-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-07-01
Question
Q: What is the optimal O.D. value for Renin ELISA kit? I performed your Renin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Renin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-03-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Renin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Renin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-03-17
Question
Q: how to thaw whole blood sample for Renin ELISA after freezing?
H. Lewis
Verified customer
Asked: 2016-04-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Renin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-04-04
