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Product Info Summary
| SKU: | EK2327 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Human RGM-A ELISA Kit PicoKine®
SKU/Catalog Number
EK2327
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human RGM-A ELISA Kit PicoKine® (96 Tests). Quantitate Human RGM-A in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human RGM-A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2327)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Cys48-Gly422 & Pro169-Gly422
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<15 pg/mL
Assay Range
125.0-8,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2327 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2327 is reactive to RGMA in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2327 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of RGMA
This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2327-CAP | Anti-Human RGMA Pre-coated 96-well strip microplate | 1 |
| EK2327-ST | Human RGMA Standard | 2 vials, 10 ng/tube |
| EK2327-DA | Human RGMA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.008 | 0.083 | 0.154 | 0.281 | 0.486 | 0.889 | 1.486 | 2.31 |
Data Example Images
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Human RGM-A PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect RGMA, Dilution ratio of 1:1, concentration in serum and plasma is around 1476-4368 pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human RGM-A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 176 | 891 | 3825 | 148 | 616 | 2759 |
| Standard deviation | 12.88 | 66.02 | 118.58 | 10.03 | 15.95 | 156.44 |
| CV (%) | 7.3% | 7.4% | 3.1% | 6.8% | 2.6% | 5.7% |
Specific Publications For Human RGM-A ELISA Kit PicoKine® (EK2327)
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