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Product Info Summary
| SKU: | EK1568 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human S100A13 ELISA Kit PicoKine®
SKU/Catalog Number
EK1568
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human S100A13 ELISA Kit PicoKine™ (96 Tests). Quantitate Human S100A13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human S100A13 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1568)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-K98
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1568 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1568 is reactive to S100A13 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1568 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of S100A13
S100 calcium-binding protein A13 (S100A13) is a protein that in humans is encoded by the S100A13 gene. The characterization of a human gene, S100A13 encodes a calcium-binding protein belonging to the S100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1568-CAP | Anti-Human S100A13 Pre-coated 96-well strip microplate | 1 |
| EK1568-ST | Human S100A13 Standard | 2 vials, 10 ng/tube |
| EK1568-DA | Human S100A13 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.034 | 0.158 | 0.246 | 0.402 | 0.633 | 1.099 | 1.701 | 2.314 |
Data Example Images
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Human S100A13 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect S100A13, Dilution ratio of 1:1, concentration in serum and plasma is around 70 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human S100A13 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 38 | 138 | 472 | 38 | 130 | 466 |
| Standard deviation | 1.78 | 5.79 | 29.73 | 1.9 | 6.63 | 34.95 |
| CV (%) | 4.7% | 4.2% | 7.5%%% | 5% | 7.5%% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of S100A13 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 38 | 38 | 36 | 38 | 37 | 0.86 | 2.3% |
| Sample 2 | 138 | 140 | 138 | 152 | 142 | 5.83 | 4.1% |
| Sample 3 | 472 | 455 | 478 | 440 | 461 | 14.88 | 3.2% |
Protein Target Info & Infographic
Gene/Protein Information For S100A13 (Source: Uniprot.Org, NCBI)
Gene Name
S100A13
Full Name
Protein S100-A13
Weight
11.471kDa
Superfamily
S-100 family
Alternative Names
S100 calcium binding protein A13; S100 calcium-binding protein A13protein S100-A13; S100A13 S100A13 S100 calcium binding protein A13 protein S100-A13
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on S100A13, check out the S100A13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for S100A13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human S100A13 ELISA Kit PicoKine® (EK1568)
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Human S100A13 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated S100A13 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the S100A13 antigen?
Verified Customer
Verified customer
Asked: 2020-01-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-01-19
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-28
