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Product Info Summary
| SKU: | EK1339 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®
SKU/Catalog Number
EK1339
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC1 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1339)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q23-G254
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1339 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1339 is reactive to SDC1 in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK1339 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SDC1
Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1339-CAP | Anti-Human SDC1 Pre-coated 96-well strip microplate | 1 |
| EK1339-ST | Human SDC1 Standard | 2 vials, 20 ng/tube |
| EK1339-DA | Human SDC1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.011 | 0.085 | 0.184 | 0.263 | 0.523 | 0.944 | 1.558 | 2.173 |
Data Example Images
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Human SDC1/Syndecan-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SDC1, Dilution ratio of 1:1, concentration in serum is 68-5651 pg/ml..
Some articles we found to cite concentrations of SDC1 in samples: 15089762 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 632 | 25676 | 8396 | 676 | 2617 | 8785 |
| Standard deviation | 26.51 | 171.27 | 545.74 | 31.09 | 196.27 | 588.59 |
| CV (%) | 4.1% | 6.6% | 6.5% | 4.6% | 7.5% | 6.7% |
Specific Publications For Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® (EK1339)
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Customer Reviews
0 Reviews For Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®
Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-11-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-11-14
Question
I did not get any measurable Syndecan-1 signal in my cell culture supernatants on ELISA but I expected some baseline signal even if it was small. Meanwhile, the standards worked fine, and I also confirmed cell activation by flow cytometry with ICAM-1. What happened? Any troubleshooting suggestions?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Confirmed cell activation doesn't indicate the sample contains protein of interest. The protein may also be in small quantities beyond the ELISA detection range. To troubleshoot, we suggest that you try to increase the cell density and/or homogenize/lyse the cells in lysis buffer (instead of working with cell supernatant).
Boster Scientific Support
Answered: 2020-05-01
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-01-13
Question
Q: What is the optimal O.D. value for CD138 ELISA kit? I used your CD138 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD138 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-10-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD138 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD138 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-10-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-26
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
J. Zhou
Verified customer
Asked: 2018-05-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-25
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD138 using the EK1339 Human CD138 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-16
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD138 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2014-09-21
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD138 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-09-21
