Human SEZ6L ELISA Kit PicoKine™

Human SEZ6L ELISA Kit PicoKine™ (96 Tests). Quantitate Human SEZ6L in cell culture supernatants, serum and plasma (heparin). Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1862
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human SEZ6L ELISA Kit PicoKine™

See all SEZ6L products

SKU/Catalog Number

EK1862

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SEZ6L ELISA Kit PicoKine™ (96 Tests). Quantitate Human SEZ6L in cell culture supernatants, serum and plasma (heparin). Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human SEZ6L ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1862)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-I1024

Sensitivity

<0.1 ng/ml

Assay Range

0.312 ng/ml - 20 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1862 is reactive to SEZ6L in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1862 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1862-CAP Anti-Human SEZ6L Pre-coated 96-well strip microplate 1
EK1862-ST Human SEZ6L Standard 2 vials, 20 ng/tube
EK1862-DA Human SEZ6L Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0312.56251250250050001000020000
O.D.0.0410.1410.1930.3410.5980.8521.5312.256

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SEZ6L, Dilution ratio of 1:1, concentration in serum is around 9.6ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SEZ6L ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)489173410155544201211046
Standard deviation31.3097.10710.8523.39114.68839.50
CV (%)6.4%5.6%7.0%4.3%5.7%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SEZ6L in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 148947851752450219.063.7%
Sample 21734175619061797179866.193.6%
Sample 3101558845976689179420557.575.9%
*number of samples for each test n=16.

Gene/Protein Information For SEZ6L (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BYH1

Gene ID

23544

Gene Name

SEZ6L

Full Name

Seizure 6-like protein

Weight

111.782kDa

Superfamily

SEZ6 family

Alternative Names

Acig1; AIG1; Aig1L; BSRP-B; seizure related 6 homolog (mouse)-like; seizure related gene 6-like; SEZ6L SEZ6L seizure related 6 homolog like seizure 6-like protein|seizure related gene 6-like

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SEZ6L, check out the SEZ6L Infographic

SEZ6L infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SEZ6L: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1862

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10 Customer Q&As for Human SEZ6L ELISA Kit PicoKine™

Question

Q: Can SEZ6L ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-12-09

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-12-09

Question

Q: how to analyze ELISA data? I have performed SEZ6L level in plasma.

Verified Customer

Verified customer

Asked: 2020-06-13

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-06-13

Question

Q: What is the optimal O.D. value for SEZ6L ELISA kit? I performed your SEZ6L ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain SEZ6L even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SEZ6L ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of SEZ6L that can be declared/interpreted as positive by the above standard is 0.1ng/ml.

Boster Scientific Support

Answered: 2019-05-16

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-02-21

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-21

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-01-01

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-01-01

Question

Q: how to thaw whole blood sample for SEZ6L ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SEZ6L for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-12-27

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-12-17

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-12-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Walker

Verified customer

Asked: 2017-12-04

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-12-04

Question

A customer wanted to know the appropriate dilution for normal serum when using EK1862.

Verified Customer

Verified customer

Asked: 2017-06-06

Answer

The suggested dilution ratio for normal serum extracted from healthy human is around 1:4. Please run a pilot test to figure out the optimal dilution.

Boster Scientific Support

Answered: 2017-06-06

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring SEZ6L using the EK1862 Human SEZ6L Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-01-12

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2017-01-12

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