Product Info Summary
SKU: | FEK0503 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
Assay Time: | 90 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human sL-Selectin PicoKine® Quick ELISA Kit
See all L-Selectin/CD62L products
SKU/Catalog Number
FEK0503
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human sL-Selectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SELL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human sL-Selectin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0503)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: W39-N332
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0503 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0503 is reactive to SELL in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
FEK0503 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of L-Selectin/CD62L
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Kit Components
Catalog number | Description | Quantity | Volume |
---|---|---|---|
FEK0503-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
EK0503-ST | Human L-Selectin/CD62L Standard | 2 | 10 ng/tube |
FEK0503-A | Human SELL Antibody A | 1 | 3ml |
FEK0503-B | Human SELL Antibody B | 1 | 3ml |
AR1106-1 | Sample Diluent | 1 | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
AR1105 | Stop Solution | 1 | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.011 | 0.262 | 0.446 | 0.852 | 1.492 | 2.205 | 2.476 | 2.617 |
Data Example Images
Click image to see more details
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1187 | 543 | 1239 | 187 | 595 | 1352 |
Standard deviation | 17.8%1 | 33.66 | 60.71 | 16.64 | 37.48 | 67.6 |
CV (%) | 7.8% | 6.2% | 4.9% | 8.9% | 6.3% | 5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of L-Selectin/CD62L in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 195 | 209 | 190 | 190 | 196 | 7.77 | 3.9% |
Sample 2 | 543 | 572 | 564 | 574 | 563 | 12.27 | 2.1% |
Sample 3 | 1239 | 1413 | 1184 | 1268 | 1276 | 84.65 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For SELL (Source: Uniprot.Org, NCBI)
Gene Name
SELL
Full Name
L-selectin
Weight
42.187kDa
Superfamily
selectin/LECAM family
Alternative Names
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1 SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 antigen-like family member L|gp90-MEL|leukocyte surface antigen Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SELL, check out the SELL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SELL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human sL-Selectin PicoKine® Quick ELISA Kit (FEK0503)
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