Anti-CD62L/SELL Antibody Picoband®

Product Name

Anti-CD62L/SELL Antibody Picoband®

View all Sell Antibodies

SKU/Catalog Number

PA1359
BA4555-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

SELL (L-selectin/CD62L) is a selectin-family adhesion receptor that mediates leukocyte tethering/rolling on endothelium and supports lymphocyte homing (GO: leukocyte adhesion/rolling); ectodomain shedding is part of regulated trafficking (e.g., by ADAM17) (disease linkage: inflammatory cell recruitment, putative). Assay context: validated for WB in mouse/rat; often interpreted alongside B-cell/immune activation markers such as BAFF (TNFSF13B) and CD40 (and the CD40L/CD40LG axis).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD62L/SELL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1359)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse L-selectin, identical to the related rat sequence.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1359 is reactive to Sell in Mouse, Rat

Observed Molecular Weight

70 kDa

Calculated molecular weight

42.3 kDa

Background of Sell

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1359 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: Rat Spleen Tissue, Mouse Spleen Tissue

Validation Images & Assay Conditions

See full target information Sell

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