|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-L-Selectin Antibody|
|Description||Rabbit IgG polyclonal antibody for L-selectin(SELL) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-L-Selectin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1359)|
|Replacement Item||This antibody may replace the following items: sc-13505|sc-15331|sc-18851|sc-18905|sc-18918|sc-390756|sc-6946|sc-71476 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse L-selectin (125-141aa KEDCVEIYIKRERDSGK), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||42288 MW|
|Protein Function||Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity). .|
|Tissue Specificity||Predominantly expressed in lymphoid tissue. .|
|Sequence Similarities||Belongs to the selectin/LECAM family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;Lymphocyte antigen 22;Ly-22;Lymphocyte surface MEL-14 antigen;CD62L;Sell;Lnhr, Ly-22, Ly22;|
|Research Areas|||cardiovascular|blood| immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage|myeloid|neutrophil lineage|regulatory t cells||
Background for L-selectin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-L-Selectin Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-L-Selectin Antibody Images
Click the images to enlarge.
All lanes: Anti L-selectin (PA1359) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 70KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,