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Product Info Summary
| SKU: | FEK0771 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human SPINT1/HAI-1 PicoKine® Quick ELISA Kit
See all HAI-1/HGFA Inhibitor 1 products
SKU/Catalog Number
FEK0771
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SPINT1/HAI-1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SPINT1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SPINT1/HAI-1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0771)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G36-L529
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0771 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0771 is reactive to SPINT1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
FEK0771 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HAI-1/HGFA Inhibitor 1
Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0771-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0771-ST | Human HAI-1/HGFA Inhibitor 1 Standard | 2 | 10 ng/tube |
| FEK0771-A | Human SPINT1 Antibody A | 1 | 3ml |
| FEK0771-B | Human SPINT1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.036 | 0.102 | 0.150 | 0.243 | 0.421 | 0.765 | 1.383 | 2.228 |
Data Example Images
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Human SPINT1/HAI-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 211 | 1855 | 3887 | 234 | 1852 | 3784 |
| Standard deviation | 13.5 | 116.87 | 264.32 | 17.55 | 120.38 | 298.94 |
| CV (%) | 6.4% | 6.3% | 6.8% | 7.5% | 6.5% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HAI-1/HGFA Inhibitor 1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 211 | 244 | 208 | 233 | 224 | 12.32 | 5.5% |
| Sample 2 | 1855 | 1675 | 1694 | 1706 | 1733 | 39.85 | 2.3% |
| Sample 3 | 3887 | 4423 | 4263 | 4699 | 4318 | 250.44 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For SPINT1 (Source: Uniprot.Org, NCBI)
Gene Name
SPINT1
Full Name
Kunitz-type protease inhibitor 1
Weight
58.398kDa
Alternative Names
HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1 SPINT1 HAI, HAI1, MANSC2 serine peptidase inhibitor, Kunitz type 1 kunitz-type protease inhibitor 1|HAI-1|hepatocyte growth factor activator inhibitor type 1|serine protease inhibitor, Kunitz type 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SPINT1, check out the SPINT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SPINT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SPINT1/HAI-1 PicoKine® Quick ELISA Kit (FEK0771)
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