Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human STC2 in cell culture supernatants and serum. Sensitivity: 5pg/ml.

Product Info Summary

SKU: EK1988
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and serum

Product Name

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™

See all Stanniocalcin 2/STC-2 products

SKU/Catalog Number

EK1988

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human STC2 in cell culture supernatants and serum. Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1988)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-R302

Sensitivity

<5 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1988 is reactive to STC2 in Human samples

Validated Sample Types

cell culture supernatants and serum

Application Guarantee

EK1988 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1988-CAP Anti-Human STC2 Pre-coated 96-well strip microplate 1
EK1988-ST Human STC2 Standard 2 vials, 10 ng/tube
EK1988-DA Human STC2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.262.5125250500
O.D.0.0330.0820.1350.2140.4020.7311.2882.162

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Stanniocalcin 2/STC-2, Dilution ratio of 1:1, concentration in serum is 15-70pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9712071275219
Standard deviation0.383.6211.390.824.4313.36
CV (%)4.2%5.1%5.5%6.8%5.9%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Stanniocalcin 2/STC-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 191091090.505.5%
Sample 271726164674.636.9%
Sample 32072172262082147.693.5%
*number of samples for each test n=16.

Gene/Protein Information For STC2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O76061

Gene ID

8614

Gene Name

STC2

Full Name

Stanniocalcin-2

Weight

33.249kDa

Superfamily

stanniocalcin family

Alternative Names

Stanniocalcin 2; stanniocalcin-2; STC2; STC-2; STC-2Stanniocalcin-related protein; STC-related protein; STCRP STC2 STC-2, STCRP stanniocalcin 2 stanniocalcin-2|STC-related protein|stanniocalcin-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on STC2, check out the STC2 Infographic

STC2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for STC2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1988

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine™

Question

Q: how do I analyze ELISA data? I have performed Stanniocalcin 2 level in serum.

D. Sunder

Verified customer

Asked: 2020-02-04

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-04

Question

Q: Can Stanniocalcin 2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-10-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-19

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Stanniocalcin 2 using the EK1988 Human Stanniocalcin 2 Picokine® ELISA Kit?

W. Huang

Verified customer

Asked: 2019-07-28

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-07-28

Question

Q: if the enzyme conjugated Stanniocalcin 2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Stanniocalcin 2 antigen?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-22

Question

Q: can you recommend the dilution ratio of serum samples for detection of Stanniocalcin 2 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-10-11

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Stanniocalcin 2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-10-11

Question

Q: how to thaw whole blood sample for Stanniocalcin 2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-05-02

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Stanniocalcin 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-05-02

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1988
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.