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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human TAFI/CPB2/Carboxypeptidase B2 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TAFI/CPB2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CPB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human TAFI/CPB2/Carboxypeptidase B2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1502)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M1-V423
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1502 is reactive to CPB2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1502 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1502-CAP||Anti-Human CPB2 Pre-coated 96-well strip microplate||1|
|EK1502-ST||Human CPB2 Standard||2 vials, 10 ng/tube|
|EK1502-DA||Human CPB2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TAFI/CPB2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carboxypeptidase B2/CPB2, Dilution ratio of 1:2000, concentration in serum is 1.3-3ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TAFI/CPB2/Carboxypeptidase B2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Carboxypeptidase B2/CPB2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CPB2 (Source: Uniprot.Org, NCBI)
peptidase M14 family
carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); Carboxypeptidase B2; Carboxypeptidase U; CPB2; CPU; CPUEC 184.108.40.206; EC 3.4.17; PCPB; Plasma carboxypeptidase B; TAFI; TAFIcarboxypeptidase B-like protein; Thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor CPB2 CPU, PCPB, TAFI carboxypeptidase B2 carboxypeptidase B2|carboxypeptidase B-like protein|carboxypeptidase B2 (plasma)|carboxypeptidase B2 (plasma, carboxypeptidase U)|carboxypeptidase R|thrombin-activable fibrinolysis inhibitor|thrombin-activatable fibrinolysis inhibitor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CPB2, check out the CPB2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CPB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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