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Product Info Summary
| SKU: | EK1234 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human TFF3/ITF ELISA Kit PicoKine®
SKU/Catalog Number
EK1234
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TFF3/Itf ELISA Kit PicoKine™ (96 Tests). Quantitate Human TFF3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TFF3/ITF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1234)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E22-Y80
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1234 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1234 is reactive to TFF3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK1234 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TFF3
Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1234-CAP | Anti-Human TFF3 Pre-coated 96-well strip microplate | 1 |
| EK1234-ST | Human TFF3 Standard | 2 vials, 10 ng/tube |
| EK1234-DA | Human TFF3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.041 | 0.107 | 0.181 | 0.321 | 0.591 | 1.128 | 1.429 | 2.090 |
Data Example Images
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Human TFF3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TFF3, Dilution ratio of 1:1, concentration in serum and plasma is 1-2 ng/ml..
Some articles we found to cite concentrations of TFF3 in samples: 25031551 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TFF3/ITF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 92 | 646 | 1668 | 89 | 679 | 1553 |
| Standard deviation | 5.06 | 41.34 | 78.39 | 6.4%9 | 51.6 | 100.94 |
| CV (%) | 5.5% | 6.4% | 4.7% | 6.4% | 7.6% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TFF3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 92 | 86 | 91 | 87 | 89 | 2.54 | 2.8% |
| Sample 2 | 646 | 631 | 687 | 654 | 654 | 20.5 | 3.1% |
| Sample 3 | 1668 | 1662 | 1800 | 1892 | 1755 | 96.19 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For TFF3 (Source: Uniprot.Org, NCBI)
Gene Name
TFF3
Full Name
Trefoil factor 3
Weight
10.181kDa
Alternative Names
HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal) TFF3 ITF, P1B, TFI trefoil factor 3 trefoil factor 3|polypeptide P1.B|trefoil factor 3 (intestinal)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TFF3, check out the TFF3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TFF3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TFF3/ITF ELISA Kit PicoKine® (EK1234)
Hello CJ!
EK1234 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tapias NS,Denadai-Souza A,Rolland-Fourcade C,Quaranta-Nicaise M,Blanpied C,Marcellin M,Edir A,Rolland C,Cirillo C,Dietrich G,Alric L,Portier G,Kirzin S,Bonnet D,Mas E,Burlet-Schiltz O,Deraison C,Bonnart C,Vergnolle N,Barreau F.Colitis-linked to endoplasmi
Species: Human
EK1234 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human TFF3/ITF ELISA Kit PicoKine®
Question
Q: Can TFF3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Krishna
Verified customer
Asked: 2020-05-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-05-23
Question
Q: is it okay to use citrate plasma as samples in Human TFF3 Picokine® ELISA Kit (Catalog # EK1234)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TFF3 causing degradation of its protein structure. TFF3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TFF3 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-01
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-10
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
A. Green
Verified customer
Asked: 2019-03-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-25
Question
Q: how can I thaw whole blood sample for TFF3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-12-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TFF3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-12-02
Question
Q: if the enzyme conjugated TFF3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TFF3 antigen?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-10-19
Question
Q: can you recommend the dilution ratio of serum samples for detection of TFF3 in Human saliva? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2014-10-06
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TFF3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-10-06
