Product Info Summary
SKU: | EK7098 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
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Product info
Product Name
Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit
SKU/Catalog Number
EK7098
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Thyroid-Stimulating Hormone (TSH). 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7098)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.10 mIU/L
Assay Range
0.3-12 mIU/L
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7098 is reactive to TSHR in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7098 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Anti-Human Thyroid-Stimulating Hormone (TSH) Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti- Thyroid-Stimulating Hormone (TSH) monoclonal antibody, Polystyrene micro-well plate |
Human Thyroid-Stimulating Hormone (TSH) Standards (S0~S6) | 7 | 1ml | Thyroid-Stimulating Hormone (TSH) (0, 0.3, 0.6, 1.5, 3.0, 6.0, 12.0mIU/L), 0.02M PBS, 2% BSA, 0.1%Proclin-300 |
HRP Conjugated anti-Human Thyroid-Stimulating Hormone (TSH) antibody | 1 | 6ml | HRP Conjugated anti-Human Thyroid-Stimulating Hormone (TSH) antibody, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, 0.01% azophloxine |
Controls | 2 | 1ml | Thyroid-Stimulating Hormone (TSH), 100% natural protein, 0.1%Proclin-300 |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Human TSH ELISA Kit standard curve
Protein Target Info & Infographic
Gene/Protein Information For TSHR (Source: Uniprot.org, NCBI)
Gene Name
TSHR
Full Name
Thyrotropin receptor
Weight
86.83kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
CHNG1; LGR3; LGR3hTSHR-I; MGC75129; seven transmembrane helix receptor; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH R; TSHR; TSH-R TSHR CHNG1, LGR3, hTSHR-I thyroid stimulating hormone receptor thyrotropin receptor|TSH receptor|seven transmembrane helix receptor|thyrotropin receptor-I, hTSHR-I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSHR, check out the TSHR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSHR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit (EK7098)
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