Human Thyroxine (T4) ELISA Kit

Product Name

Human Thyroxine (T4) ELISA Kit

SKU/Catalog Number

EK7012

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human T4. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Thyroxine (T4) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7012)

Clonality of Antibodies

See Datasheet for details

Sensitivity

15 ng/mL

Assay Range

20-320 ng/mL

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7012 is reactive to in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7012 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Rabbit Anti- T4 Antibody, Polystyrene micro-well plate
Human T4 Standards (S0~S5)	5	1ml	T4 (0, 20, 40, 80, 160, 320 ng/mL), 100% detoxification serum, 0.1% Proclin-300
Avidin-HRP conjugate	1	6ml	Avidin-HRP conjugate, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1% Proclin-300
Biotinylated antigen	1	6ml	Biotinylated T4, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300
Controls	2	1ml	T4, 100% natural protein, 0.1% Proclin-300 Range of Control 1: 44.3-82.3 ng/mL Range of Control 2: 112-208 ng/mL
20X Wash Buffer Concentrate	1	15ml	0.2M PBS containing 0.5% tween 20
Color Developing Reagent A	1	7ml	11m mol/L Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution	1	7ml	2mol/L Sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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