Human Tissue Factor/F3 ELISA Kit PicoKine™

Human Tissue Factor/F3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine. Sensitivity: 10pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0928
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.

Product Name

Human Tissue Factor/F3 ELISA Kit PicoKine™

See all Coagulation Factor III/Tissue Factor products

SKU/Catalog Number

EK0928

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Tissue Factor/F3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Tissue Factor/F3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0928)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S33-E251

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0928 is reactive to F3 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.

Application Guarantee

EK0928 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0928-CAP Anti-Human F3 Pre-coated 96-well strip microplate 1
EK0928-ST Human F3 Standard 2 vials, 10 ng/tube
EK0928-DA Human F3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D0.0310.1010.1650.2960.7080.9341.8512.431

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Coagulation Factor III/Tissue Factor, Dilution ratio of 1:1, concentration in serum is around 40pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Tissue Factor/F3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2820445528205493
Standard deviation1.2613.4634.581.5414.1440.91
CV (%)4.5%6.6%7.6%5.5%6.9%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor III/Tissue Factor in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128293127281.475.2%
Sample 220423120120320912.315.8%
Sample 345542538943142523.625.5%
*number of samples for each test n=16.

Gene/Protein Information For F3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P13726

Gene ID

2152

Gene Name

F3

Full Name

Tissue factor

Weight

33.068kDa

Superfamily

tissue factor family

Alternative Names

CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on F3, check out the F3 Infographic

F3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for F3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0928 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cohen, J.G., Prendergast, E., Geddings, J.E., Walts, A.E., Agadjanian, H., Hisada, Y.,…& Walsh, C.S. (2017). Evaluation of venous thrombosis and tissue factor in epithelial ovarian cancer. Gynecologic Oncology. Advance online publication. doi: 10....

Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua Zc. Mol Cell Proteomics. 2011 Jun;10(6):M111.009399. Doi: 10.1074/Mcp.M111.009399. Epub 2011 Apr 7. Proteomic Screening Of Anaerobically Reg...

Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.

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10 Customer Q&As for Human Tissue Factor/F3 ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for Tissue Factor ELISA after freezing?

M. Williams

Verified customer

Asked: 2020-12-06

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Tissue Factor for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-12-06

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-11-12

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-11-12

Question

Can we measure the concentration of recombinant tissue factor, for both natural and recombinant?

Verified customer

Asked: 2020-06-09

Answer

The expression system of standard (recombinant protein) was NSO eukaryotic expression system. If the recombinant tissue factor was expressed using eukaryotic expression system, the Human Tissue Factor/F3 ELISA Kit PicoKine™ (EK0928) will be more likely to detect it. If the recombinant tissue factor was expressed using prokaryotic expression system, the kit might be less likely to detect it. We suggest the customer run pilot test to see if the kit will work.

Boster Scientific Support

Answered: 2020-06-17

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-12-17

Question

Q: can you suggest the dilution ratio of serum samples for detection of Tissue Factor in Human Cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

W. Kulkarni

Verified customer

Asked: 2019-06-09

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Tissue Factor ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-02-27

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-27

Question

Q: how to proceed with the analysis of ELISA data? I have performed Tissue Factor level in Cell lysates.

Verified Customer

Verified customer

Asked: 2019-01-29

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-29

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-11-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-11-19

Question

Q: if the enzyme conjugated Tissue Factor antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Tissue Factor antigen?

R. Parker

Verified customer

Asked: 2018-04-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-04-16

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Tissue Factor using the EK0928 Human Tissue Factor Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-01-15

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-01-15

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