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Product Info Summary
| SKU: | FEK1226 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human TNFRSF14/HVEM PicoKine® Quick ELISA Kit
See all HVEM/TNFRSF14 products
SKU/Catalog Number
FEK1226
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF14/HVEM PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TNFRSF14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFRSF14/HVEM PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1226)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L39-V202
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1226 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1226 is reactive to TNFRSF14 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1226 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HVEM/TNFRSF14
Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
Kit Components
| Catalog number | Description | Quantity |
|---|---|---|
| FEK1226-CAP | 96-well plate precoated with anti-Human HVEM/TNFRSF14 antibody | 1 |
| EK1226-ST | lyophilized recombinant Human HVEM/TNFRSF14 standard | 10 ng/tube |
| FEK1226-DA | Biotinylated anti-Human HVEM/TNFRSF14 detection antibody (50x) | 100 ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30 ml |
| AR1106-2 | Antibody diluent | 12 ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12 ml |
| AR1106-5 | Wash Buffer (25x) | 20 ml |
| AR1104 | Color Developing Reagent (TMB) | 10 ml |
| AR1105 | Stop Solution | 10 ml |
| PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.029 | 0.092 | 0.148 | 0.267 | 0.477 | 0.910 | 1.518 | 2.294 |
Data Example Images
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Human TNFRSF14/HVEM PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 202 | 389 | 32 | 205 | 420 |
| Standard deviation | 1.58 | 5.8%8 | 23.34 | 1.85 | 11.89 | 27.3 |
| CV (%) | 4.8% | 4.1% | 6% | 5.8% | 5.8% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HVEM/TNFRSF14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 32 | 33 | 31 | 32 | 0.82 | 2.5% |
| Sample 2 | 202 | 207 | 234 | 232 | 218 | 14.37 | 6.5% |
| Sample 3 | 389 | 428 | 413 | 386 | 404 | 17.36 | 4.2% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF14 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q92956
Gene ID
8764
Gene Name
TNFRSF14
Full Name
Tumor necrosis factor receptor superfamily member 14
Weight
30.392kDa
Superfamily
tumor necrosis factor receptor superfamily
Alternative Names
ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2 TNFRSF14 ATAR, CD270, HVEA, HVEM, LIGHTR, TR2 TNF receptor superfamily member 14 tumor necrosis factor receptor superfamily member 14|CD40-like protein|herpes virus entry mediator A|tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)|tumor necrosis factor receptor-like gene2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF14, check out the TNFRSF14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFRSF14/HVEM PicoKine® Quick ELISA Kit (FEK1226)
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