This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
TNFRSF14 antibody (PB10104) performs well in ELISA, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Western blot analysis of TNFRSF14/HVEM using anti-TNFRSF14/HVEM antibody (PB10104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: SW620 whole cell lysates lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF14/HVEM antigen affinity purified polyclonal antibody (Catalog # PB10104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF14/HVEM at approximately 37 kDa. The expected band size for TNFRSF14/HVEM is at 30 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: SW620 whole cell lysates lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF14/HVEM antigen affinity purified polyclonal antibody (Catalog # PB10104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF14/HVEM at approximately 37 kDa. The expected band size for TNFRSF14/HVEM is at 30 kDa.
Bundle Save
Anti-TNFRSF14 Antibody Picoband®
TNFRSF14 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, WB |
Tested Applications
TNFRSF14 antibody was positively detected by WB in:
HELA whole cell, SW620 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
ELISA
0.1-0.5μg/ml
-
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-TNFRSF14 Antibody Picoband®
View all TNFRSF14 Antibodies
View all TNFRSF14 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TNFRSF14/HVEM recombinant protein (Position: L39-V202).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated Molecular Weight
30.4 kDa
Background of TNFRSF14
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-TNFRSF14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10104)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
88%
Moderate Homology
Dog
68%
Low homology
Mouse
55%
Low homology
Rat
54%
Low homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TNFRSF14 antibody
Review
Have you used Anti-TNFRSF14 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-TNFRSF14 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB10104
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026