Click image to see more details
Product Info Summary
| SKU: | A05206 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CLK2 Antibody
SKU/Catalog Number
A05206
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CLK2 Antibody catalog # A05206. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05206)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CLK2. AA range:1-50
Reactive Species
A05206 is reactive to CLK2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
60.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05206 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using CLK2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Click image to see more details
Western Blot analysis of A549 cells using CLK2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Click image to see more details
Western blot analysis of lysates from COLO205 and A549 cells, using CLK2 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Specific Publications For Anti-CLK2 Antibody (A05206)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CLK2 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CLK2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-CLK2 Antibody
Question
Is this A05206 anti-CLK2 antibody reactive to the isotypes of CLK2?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The immunogen of A05206 anti-CLK2 antibody is Synthesized peptide derived from the N-terminal region of human CLK2. at AA rangle: 1-80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-30
Question
Here is the WB image, lot number and protocol we used for lung ovary using anti-CLK2 antibody A05206. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
We are interested in to test anti-CLK2 antibody A05206 on rat lung ovary for research purposes, then I may be interested in using anti-CLK2 antibody A05206 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The products we sell, including anti-CLK2 antibody A05206, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-07
Question
Will anti-CLK2 antibody A05206 work on pig WB with cervix carcinoma?
D. Jackson
Verified customer
Asked: 2019-07-18
Answer
Our lab technicians have not tested anti-CLK2 antibody A05206 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CLK2 antibody A05206 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-18
Question
Do you have a BSA free version of anti-CLK2 antibody A05206 available?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CLK2 antibody A05206 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-23
Question
I see that the anti-CLK2 antibody A05206 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-06-08
Question
We are currently using anti-CLK2 antibody A05206 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
C. Johnson
Verified customer
Asked: 2015-11-06
Answer
The anti-CLK2 antibody (A05206) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-06
