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Product Info Summary
| SKU: | EK1684 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine®
View all Transthyretin/Prealbumin ELISA kits
SKU/Catalog Number
EK1684
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TTR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1684)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G21-E147
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1684 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1684 is reactive to TTR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1684 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Transthyretin/Prealbumin
Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1684-CAP | Anti-Human TTR Pre-coated 96-well strip microplate | 1 |
| EK1684-ST | Human TTR Standard | 2 vials, 20ng/tube |
| EK1684-DA | Human TTR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.4 | 125 | 250 | 500 | 1000 |
| O.D. | 0.009 | 0.059 | 0.168 | 0.352 | 0.560 | 1.104 | 1.664 | 1.897 |
Data Example Images
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Human Transthyretin/TTR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Transthyretin/Prealbumin, Dilution ratio of 1:1000000, concentration in serum and plasma is around 240 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 74 | 276 | 990 | 72 | 289 | 909 |
| Standard deviation | 3.55 | 11.04 | 71.28 | 3.67 | 16.76 | 71.81 |
| CV (%) | 4.8% | 4% | 7.2% | 7.9% | 5.8% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Transthyretin/Prealbumin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 74 | 75 | 68 | 79 | 74 | 3.85 | 5.2% |
| Sample 2 | 276 | 264 | 283 | 252 | 269 | 9.68 | 3.6% |
| Sample 3 | 990 | 955 | 987 | 908 | 960 | 18.24 | 1.9% |
Protein Target Info & Infographic
Gene/Protein Information For TTR (Source: Uniprot.Org, NCBI)
Gene Name
TTR
Full Name
Transthyretin
Weight
15.887kDa
Superfamily
transthyretin family
Alternative Names
ATTR; HsT2651; PALB; PALBTBPA; Prealbumin; prealbumin, amyloidosis type I; TBPA; thyroxine-binding prealbumin; Transthyretin; TTR TTR ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN transthyretin transthyretin|epididymis luminal protein 111|prealbumin, amyloidosis type I|thyroxine-binding prealbumin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TTR, check out the TTR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TTR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine® (EK1684)
Hello CJ!
EK1684 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cognitive Frailty in Thai Community-Dwelling Elderly: Prevalence and Its Association with Malnutrition
Wen J,Wu J,Cao T,Zhi S,Chen Y,Aagaard L,Zhen P,Huang Y,Zhong J,Huang J.Methylation silencing and reactivation of exogenous genes in lentivirus-mediated transgenic mice.Transgenic Res.2021 Jan 4.doi:10.1007/s11248-020-00224-9.Epub ahead of print.PMID:33394
Species: Mouse
EK1684 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine®
Question
Is EK1684 quantitative?
Verified customer
Asked: 2021-01-13
Answer
The Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684) is quantitative.
Boster Scientific Support
Answered: 2021-01-13
Question
Is EK1684 pre-coated?
Verified customer
Asked: 2021-01-13
Answer
The Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684) is pre-coated.
Boster Scientific Support
Answered: 2021-01-13
Question
Has EK1684 been tested/ validated on human plasma?
Verified customer
Asked: 2021-01-13
Answer
The Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684) has been tested on human plasma.
Boster Scientific Support
Answered: 2021-01-13
Question
Has EK1684 been tested/ validated on human plasma EDTA?
Verified customer
Asked: 2021-01-13
Answer
The Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684) has been tested on human plasma EDTA.
Boster Scientific Support
Answered: 2021-01-13
Question
What is the range of values (with units) measured in human plasma specifically (not serum) for EK1684?
Verified customer
Asked: 2021-01-13
Answer
For the Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684), the range of value is 100-500ug/ml.
Boster Scientific Support
Answered: 2021-01-13
Question
Do you make EK1684 yourself?
Verified customer
Asked: 2021-01-13
Answer
Yes, we make the Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684) ourselves.
Boster Scientific Support
Answered: 2021-01-13
Question
Does EK1684 measure total concentration?
Verified customer
Asked: 2021-01-13
Answer
The Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine™ (EK1684) measures total concentration.
Boster Scientific Support
Answered: 2021-01-13
Question
Q: how to analyze ELISA data? I have obtained Transthyretin level in plasma.
W. Miller
Verified customer
Asked: 2020-10-11
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-10-11
Question
Q: if the enzyme conjugated Transthyretin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Transthyretin antigen?
B. Patel
Verified customer
Asked: 2018-11-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-21
Question
Q: is it okay to use citrate plasma as samples in Human Transthyretin Picokine® ELISA Kit (Catalog # EK1684)?
W. Mangal
Verified customer
Asked: 2018-08-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Transthyretin causing degradation of its protein structure. Transthyretin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Transthyretin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-29
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Z. Zhao
Verified customer
Asked: 2018-05-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-22
Question
Q: Can Transthyretin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-01-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
A. Taylor
Verified customer
Asked: 2018-01-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-01-08
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Transthyretin using the EK1684 Human Transthyretin Picokine® ELISA Kit?
M. Kaur
Verified customer
Asked: 2014-09-29
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2014-09-29
