Human TREML1/Tlt 1 ELISA Kit PicoKine®

Product Name

Human TREML1/Tlt 1 ELISA Kit PicoKine®

View all TREML1/TLT-1 ELISA kits

SKU/Catalog Number

EK1521

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TREML1/Tlt 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TREML1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TREML1/Tlt 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1521)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q16-P162

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1521 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1521 is reactive to Treml1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1521 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TREML1/TLT-1

Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. This protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number	Description	Quantity
EK1521-CAP	Anti-Human Treml1 Pre-coated 96-well strip microplate	1
EK1521-ST	Human Treml1 Standard	2 vials, 1 ng/tube
EK1521-DA	Human Treml1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.002	0.080	0.155	0.307	0.639	1.063	1.748	2.066

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TREML1/TLT-1, Dilution ratio of 1:100, concentration in serum and plasma is around 30 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TREML1/Tlt 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TREML1/TLT-1 in ELISA kits from four different production batches/lots.

Gene/Protein Information For Treml1 (Source: Uniprot.Org, NCBI)

Alternative Names

dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1 Treml1|5430401J17Rik, TLT-, TLT-1|triggering receptor expressed on myeloid cells-like 1|trem-like transcript 1 protein|triggering receptor expressed on myeloid cells-like 1c|triggering receptor expressed on myeloid cells-like 1d|triggering receptor expressed on myeloid cells-like 1e|triggering receptor expressed on myeloid cells-like protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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