Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®

TPSAB1 ELISA kit for Human

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0898
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®

View all TPSAB1 ELISA kits

SKU/Catalog Number

EK0898

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0898)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-V275

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0898 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0898 is reactive to TPSAB1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0898 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TPSAB1

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Kit Components

Catalog Number Description Quantity
EK0898-CAP Anti-Human TPSAB1 Pre-coated 96-well strip microplate 1
EK0898-ST Human TPSAB1 Standard 2 vials, 10 ng/tube
EK0898-DA Human TPSAB1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0680.1130.2120.3010.4590.7141.2361.861

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TPSAB1, Dilution ratio of 1:1, concentration in serum and plasma is 2.5-5 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2761887560828320545485
Standard deviation18.49143.41420.623.48191.02488.16
CV (%)6.7%7.6%7.5%8.3%9.3%8.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TPSAB1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127625528427627210.753.9%
Sample 21887199119591839191959.593.1%
Sample 356085276555958245566195.243.5%
*number of samples for each test n=16.

Gene/Protein Information For TPSAB1 (Source: Uniprot.Org, NCBI)

Gene Name

TPSAB1

Full Name

Tryptase alpha/beta-1

Weight

30.515kDa

Superfamily

peptidase S1 family

Alternative Names

alpha-Tryptase; TPS1; TPS2; TPSAB1; TPSB1; Tryptase 1; Tryptase alpha; tryptase alpha/beta 1 TPSAB1 TPS1, TPS2, TPSB1, TPSB2, Tryptase-2 tryptase alpha/beta 1 tryptase alpha/beta-1|Tryptase II|Tryptase beta-2|epididymis secretory sperm binding protein|mast cell alpha II tryptase|mast cell beta I tryptase|tryptase alpha II|tryptase alpha-1|tryptase beta I|tryptase beta-1|tryptase-1|tryptase-I|tryptase-III

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TPSAB1, check out the TPSAB1 Infographic

TPSAB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TPSAB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0898 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Blood tryptase and thymic stromal lymphopoietin levels predict the risk of exacerbation in severe asthma

Shih-Lung Cheng,Ching-Hsiung Lin,Sheng-Hao Lin et al.Blood tryptase and thymic stromal lymphopoietin levels predict the risk of exacerbation in severe asthma,02 December 2020,PREPRINT (Version 1)available at Research Square [+https://doi.org/10.21203/rs.3
Species: Human
EK0898 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

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8 Customer Q&As for Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®

Question

Could EK0898 also test Alpha?

Verified customer

Asked: 2023-08-10

Answer

Yes, the Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine (EK0898) could also test Alpha.

Boster Scientific Support

Answered: 2023-08-11

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

V. Martin

Verified customer

Asked: 2020-02-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-02-06

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TPSB1 using the EK0898 Human TPSB1 Picokine® ELISA Kit?

M. Banerjee

Verified customer

Asked: 2019-10-29

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2019-10-29

Question

For EK0898, Does this kit detect all three tryptase types; A , B1 and B2 ?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

This kit detects all three tryptase types; A , B1 and B2.

Boster Scientific Support

Answered: 2019-10-08

Question

Does EK0898 have cross reactivity to mouse?

Verified customer

Asked: 2019-09-20

Answer

The Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine™ (EK0898) does not cross react with mouse.

Boster Scientific Support

Answered: 2019-09-23

Question

Q: how to thaw whole blood sample for TPSB1 ELISA after freezing?

M. Tiwari

Verified customer

Asked: 2019-03-20

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TPSB1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-03-20

Question

Q: can you suggest the dilution ratio of serum samples for detection of TPSB1 in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

D. Dhar

Verified customer

Asked: 2018-11-30

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TPSB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-11-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-01-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-01-25

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