Overview
| Product Name |
Human TSLP ELISA Kit PicoKine™
See all TSLP primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0958 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human TSLP with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. TSLP tissue specificity: Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. . |
| Cite This Product |
Human TSLP ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0958)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
31.2pg/ml-2000pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: Y29-Q159 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For TSLP (Source: Uniprot.org, NCBI)
| Uniprot Id | Q969D9 |
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| NCBI Gene Id | 85480 |
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| Species Of This Entry | Human |
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| Gene Name | TSLP |
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| Protein Name | Thymic stromal lymphopoietin |
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| Alternative Names | TSLP|thymic stromal lymphopoietin; TSLP |
|---|
| Molecular Weight | 18141 |
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| Post Tranlational Modifications | Phosphorylation; Glycosylation; Cleavage; Acetylation; Deglycosylation |
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| Subcellular Localization | Secreted. |
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| Gene Location | 5q22.1, on Chromasome 5, gene sequence: NC_000005.10 (111070080..111078026) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TSLP (Source: Uniprot.org, NCBI)
| Protein Function | Isoform 1: Cytokine that induces the release of T-cell- attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
|---|
| Tissue Specificity | Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. |
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| Research Areas | Innate Immunity |
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| Related Diseases | Inflammation; Asthma; Dermatitis, Atopic; Dermatitis; Allergy; Rhinorrhea; Allergic Asthma; Allergic Rhinitis (disorder); Lung Diseases; Dermatologic Disorders; Virus Diseases; Neoplasms; Pneumonia; Lung Diseases, Obstructive; Malignant Neoplasms; Chronic Obstructive Airway Disease; Eosinophilia; Autoimmunity; Hyperplasia; Colitis |
|---|
| Related Pathways | Pathogenesis; Immune Response; Sensitization; Cytokine Production; Secretion; Cell Development; Cell Activation; Cell Proliferation; Cell Differentiation; T Cell Homeostasis; Cytokine Secretion; Inflammatory Response; Hypersensitivity; Adaptive Immune Response; Cell Maturation; Innate Immune Response; Glycosylation; Cell Migration; T Cell Activation; Skin Development |
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| Background | Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. |
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| Scientific References | PMID: 11418668 by Reche P.A., et al. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells.
PMID: 11480573 by Quentmeier H., et al. Cloning of human thymic stromal lymphopoietin (TSLP) and signaling mechanisms leading to proliferation. |
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Kit Components And Assay QC Data Details Of Human TSLP ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0958-CAP | 96-well plate precoated with anti-Human TSLP antibody | 1 |
| EK0958-ST | lyophilized recombinant Human TSLP standard | 10ng/tube |
| EK0958-DA | biotinylated anti-Human TSLP antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0958 Human TSLP ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.072 | 0.164 | 0.267 | 0.424 | 0.714 | 1.205 | 1.786 | 2.199 |
We measured random samples of Human TSLP ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 89 | 239 | 883 | 94 | 243 | 964 |
| Standard deviation | 4.36 | 17.92 | 45.21 | 5.82 | 19.92 | 65.55 |
| CV(%) | 4.9% | 7.5% | 5.2% | 6.2% | 8.2% | 6.8% |
We measured the performance consistency of Human TSLP ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 89 | 106 | 95 | 91 | 95 | 6.57 | 6.9% |
| Sample 2 | 239 | 245 | 235 | 240 | 239 | 3.56 | 1.4% |
| Sample 3 | 883 | 960 | 834 | 974 | 912 | 57.16 | 6.2% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all TSLP primary antibodies,
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