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Human TSLP ELISA Kit PicoKine™
Human TSLP ELISA Kit PicoKine™ (96 Tests). Quantitate Human TSLP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml.
Human TSLP ELISA Kit PicoKine™ Product Summary
| SKU: | EK0958 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10 pg/ml |
| Assay Range: | 31.2 pg/ml - 2,000 pg/ml |
Overview
| Product Name | Human TSLP ELISA Kit PicoKine™
See more TSLP products |
|---|---|
| Catalog# | EK0958 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Human TSLP ELISA Kit PicoKine™ (96 Tests). Quantitate Human TSLP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product | Human TSLP ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0958) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10 pg/ml |
| Assay Range | 31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard | Expression system for standard: E.coli; Immunogen sequence: Y29-Q159 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For TSLP (Source: Uniprot.org, NCBI)
| Uniprot Id | Q969D9 |
|---|---|
| NCBI Gene Id | 85480 |
| Species Of This Entry | Human |
| Gene Name | TSLP |
| Protein Name | Thymic stromal lymphopoietin |
| Alternative Names | TSLP|thymic stromal lymphopoietin; TSLP |
| Molecular Weight | 18141 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TSLP, check out the TSLP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TSLP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Human TSLP ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0958-CAP | Anti-Human TSLP Pre-coated 96-well strip microplate | 1 |
| EK0958-ST | Human TSLP Standard | 2 vials, 10 ng/tube |
| EK0958-DA | Human TSLP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0958 Human TSLP ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.072 | 0.164 | 0.267 | 0.424 | 0.714 | 1.205 | 1.786 | 2.199 |
We measured random samples of Human TSLP ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 89 | 239 | 883 | 94 | 243 | 964 |
| Standard deviation | 4.36 | 17.92 | 45.21 | 5.82 | 19.92 | 65.55 |
| CV (%) | 4.9% | 7.5% | 5.2% | 6.2% | 8.2% | 6.8% |
We measured the performance consistency of Human TSLP ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 89 | 106 | 95 | 91 | 95 | 6.57 | 6.9% |
| Sample 2 | 239 | 245 | 235 | 240 | 239 | 3.56 | 1.4% |
| Sample 3 | 883 | 960 | 834 | 974 | 912 | 57.16 | 6.2% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Total number of citations: 0
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Questions and answers from customers related to EK0958 Human TSLP ELISA Kit PicoKine™
5 Related Questions
Question
Q: Can Tslp ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Asked: 2020-03-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-03-28
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Tslp level in plasma.
Verified Customer
Asked: 2016-12-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-12-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2016-11-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-11-22
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2016-01-23
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-23
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2016-01-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-01-17
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Domestically in USA and Canada, ships next business day if available in stock.
Contact Information
| [email protected] | |
| (888) 466-3604 |
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EK0958
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