Human VEGF PicoKine® Quick ELISA Kit

VEGFA ELISA kit, 90-minute one-step protocol

Human VEGF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Product Info Summary

SKU: FEK0539
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Assay Time: 90 Minutes

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Product Name

Human VEGF PicoKine® Quick ELISA Kit

See all VEGFA products

SKU/Catalog Number

FEK0539

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human VEGF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human VEGF PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0539)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R191

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml (human serum,plasma), 15.6 pg/ml - 1,000 pg/ml (cell culture supernates)

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0539 is reactive to VEGFA in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK0539 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of VEGFA

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

Kit Components

Catalog numberDescription QuantityVolume
FEK0539-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0539-STHuman VEGFA Standard 2 10 ng/tube
FEK0539-AHuman VEGFA Antibody A 1 3ml
FEK0539-BHuman VEGFA Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.1300.3380.5230.9121.4802.2282.4732.536

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7728095372263988
Standard deviation7.3221.8489.585.5424.7273.11
CV (%)9.5%7.8%9.4%7.7%9.4%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VEGFA in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 177818582814.966.1%
Sample 228026626425126511.944.5%
Sample 395389096399895152.315.5%
*number of samples for each test n=16.

Gene/Protein Information For VEGFA (Source: Uniprot.Org, NCBI)

Gene Name

VEGFA

Full Name

Vascular endothelial growth factor A

Weight

27.042kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VEGFA, check out the VEGFA Infographic

VEGFA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VEGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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