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Product Info Summary
| SKU: | LS010643 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) |
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Product info
Product Name
IMP3 (IGF2BP3) (NM_006547) Human Over-expression Lysate
SKU/Catalog Number
LS010643
Size
100 µg
Description
Transient overexpression lysate of insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3)
Storage & Handling
Cite This Product
IMP3 (IGF2BP3) (NM_006547) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010643)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IMP3 (IGF2BP3) Human Over-expression Lysates NM_006547.
Western blot validation of overexpression lysate (LS010643) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IGF2BP3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IGF2BP3 (Source: Uniprot.org, NCBI)
Gene Name
IGF2BP3
Full Name
Insulin-like growth factor 2 mRNA-binding protein 3
Weight
63.705kDa
Superfamily
RRM IMP/VICKZ family
Alternative Names
CT98; hKOC; IGF II mRNA binding protein 3; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; IMP-3KH domain-containing protein overexpressed in cancer; IMP3VICKZ family member 3; insulin-like growth factor 2 mRNA binding protein 3; insulin-like growth factor 2 mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KOC1DKFZp686F1078; VICKZ3cancer/testis antigen 98 IGF2BP3 CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3 insulin like growth factor 2 mRNA binding protein 3 insulin-like growth factor 2 mRNA-binding protein 3|IGF-II mRNA-binding protein 3|IGF2 mRNA-binding protein 3|KH domain containing protein overexpressed in cancer|VICKZ family member 3|cancer/testis antigen 98
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IGF2BP3, check out the IGF2BP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGF2BP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IMP3 (IGF2BP3) (NM_006547) Human Over-expression Lysate (LS010643)
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No publications found for LS010643
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