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Product Info Summary
|Description:||Transient overexpression lysate of intersectin 1 (SH3 domain protein) (ITSN1), transcript variant 1|
Intersectin 1 (ITSN1) (NM_003024) Human Over-expression Lysate
Transient overexpression lysate of intersectin 1 (SH3 domain protein) (ITSN1), transcript variant 1
Storage & Handling
Cite This Product
Intersectin 1 (ITSN1) (NM_003024) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006453)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Gene/Protein Information for ITSN1 (Source: Uniprot.org, NCBI)
11; intersectin 1 (SH3 domain protein); intersectin short variant 12; intersectin-1; ITSN; SH3 domain protein-1A; SH3 domain-containing protein 1A; Src homology 3 domain-containing protein ITSN1 ITSN, SH3D1A, SH3P17 intersectin 1 intersectin-1|SH3 domain-containing protein 1A|Src homology 3 domain-containing protein|human intersectin-SH3 domain-containing protein SH3P17|intersectin 1 (SH3 domain protein)|intersectin 1 short form variant 3|intersectin 1 short form variant, 11|intersectin short variant 12*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on ITSN1, check out the ITSN1 Infographic
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In this infographic, you will see the following information for ITSN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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