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Product Info Summary
| SKU: | LS387893 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of SET domain containing (lysine methyltransferase) 8 (SETD8) |
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Product info
Product Name
KMT5A (NM_020382) Human Over-expression Lysate
SKU/Catalog Number
LS387893
Size
100 µg
Description
Transient overexpression lysate of SET domain containing (lysine methyltransferase) 8 (SETD8)
Storage & Handling
Cite This Product
KMT5A (NM_020382) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS387893)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KMT5A Human Over-expression Lysates NM_020382.
Western blot validation of overexpression lysate (LS387893) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KMT5A (Myc-DDK-tagged) Human lysine methyltransferase 5A (KMT5A)
Protein Target Info & Infographic
Gene/Protein Information for Kmt5a (Source: Uniprot.org, NCBI)
Gene Name
Kmt5a
Full Name
N-lysine methyltransferase KMT5A
Weight
38.845kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
H4-K20-HMTase SETD8; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase SETD8; KMT5A SET domain-containing protein 8; KMT5A; Lysine N-methyltransferase 5A; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PRSET7; PR-Set7EC 2.1.1.43; SET domain containing (lysine methyltransferase) 8; SET07SET8H4-K20-specific histone methyltransferase; SET8 Kmt5a|2410195B05Rik, AA617402, AW536475, PR-S, PR-SET7, PR/SET07, Setd, Setd8|lysine methyltransferase 5A|N-lysine methyltransferase KMT5A|H4-K20-HMTase KMT5A|H4-K20-HMTase SETD8|N-lysine methyltransferase SETD8|PR/SET domain-containing protein 07|SET domain containing (lysine methyltransferase) 8|SET domain-containing protein 8|histone-lysine N-methyltransferase KMT5A|histone-lysine N-methyltransferase SETD8|lysine-specific methylase 5A
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Kmt5a, check out the Kmt5a Infographic
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In this infographic, you will see the following information for Kmt5a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KMT5A (NM_020382) Human Over-expression Lysate (LS387893)
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